Transport of the Glucosamine-Derived Browning Product Fructosazine (Polyhydroxyalkylpyrazine) Across the Human Intestinal Caco-2 Cell Monolayer: Role of the Hexose Transporters
Transport of the Glucosamine-Derived Browning Product Fructosazine (Polyhydroxyalkylpyrazine) Across the Human Intestinal Caco-2 Cell Monolayer: Role of the Hexose Transporters
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DOI：
10.1021/acs.jafc.7b01611
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发表时间：
2017-06-14
影响因子：
6.1
通讯作者：
Betti, Mirko
中科院分区：
文献类型：
--
作者：
Bhattacherjee, Abhishek;Hrynets, Yuliya;Betti, Mirko
利用Caco-2细胞模型研究了葡萄糖胺自缩合产物果糖嗪的转运机制。通过测量穿过Caco-2细胞的双向渗透系数来评估果糖嗪转运。使用根皮苷（钠依赖性葡萄糖协同转运蛋白（SGLT）1和2的抑制剂）、根皮素和槲皮素（葡萄糖转运蛋白（GLUT）1和2的抑制剂）、转胞吞抑制剂渥曼青霉素和间隙连接破坏剂细胞松弛素D评价转运机制。使用下调或过表达的细胞系进一步研究己糖转运蛋白的作用。果糖嗪的表观渗透率（P-a，P-b）为1.30 +/- 0.02 × 10（-6）cm/s。渥曼青霉素和细胞松弛素D对果糖嗪转运无显著影响（p > 0.05）。根皮苷，根皮素，槲皮素的存在下，果糖嗪运输减少。下调的GLUT细胞系不能转运果糖嗪。在人肠上皮Caco-2细胞中，GLUT 1或GLUT 2和SGLT主要负责果糖嗪转运。
The transport mechanism of fructosazine, a glucosamine self-condensation product, was investigated using a Caco-2 cell model. Fructosazine transport was assessed by measuring the bidirectional permeability coefficient across Caco-2 cells. The mechanism of transport was evaluated using phlorizin, an inhibitor of sodium-dependent glucose cotransporters (SGLT) 1 and 2, phloretin and quercetin, inhibitors of glucose transporters (GLUT) 1 and 2, transcytosis inhibitor wortmannin, and gap junction disruptor cytochalasin D. The role of hexose transporters was further studied using downregulated or overexpressed cell lines. The apparent permeability (P-a,P-b) of fructosazine was 1.30 +/- 0.02 x 10(-6) cm/s. No significant (p > 0.05) effect was observed in fructosazine transport by adding wortmannin and cytochalasin D. The presence of phlorizin, phloretin, and quercetin decreased fructosazine transport. The downregulated GLUT cells line was unable to transport fructosazine. In human intestinal epithelial Caco-2 cells, GLUT1 or GLUT2 and SGLT are mainly responsible for fructosazine transport.