Microsphere cytometry to interrogate microenvironment-dependent cell signaling.
Microsphere cytometry to interrogate microenvironment-dependent cell signaling.
复制标题
微球细胞术用于询问微环境依赖性细胞信号传导。
DOI：
10.1039/c6ib00207b
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Lorens,JamesB
中科院分区：
文献类型：
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作者：
Ertsås,HenrietteChristie;Nolan,GarryP;LaBarge,MarkA;Lorens,JamesB
Microenvironmental cues comprising surface-mediated and soluble factors control cellular signaling mechanisms underlying normal cellular responses that define homeostatic and diseased cell states. In order to measure cell signaling in single adherent cells, we developed a novel microsphere-based flow cytometry approach. Single normal or neoplastic cells were adhered to uniform microspheres that display mimetic-microenvironments comprising surface combinations of extracellular matrix (ECM) in the presence of soluble agonists/antagonists. Temporal signaling responses were measured with fluorophore-conjugated antibodies that recognize response-dependent epitopes by multiparametric flow cytometry. Using this approach we demonstrate that microenvironment-mimetic combinations of growth factors and extracellular matrix proteins generate distinct cellular signal networks that reveal unique cell signatures in normal and patient biopsy-derived neoplastic cells.
