Dissociation between vascular endothelial growth factor receptor-2 and blood vessel density in the caudate nucleus after chronic hydrocephalus
Dissociation between vascular endothelial growth factor receptor-2 and blood vessel density in the caudate nucleus after chronic hydrocephalus
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DOI：
10.1038/jcbfm.2009.98
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发表时间：
2009-11-01
影响因子：
6.3
通讯作者：
Luciano, Mark G.
中科院分区：
文献类型：
--
作者：
Deshpande, Abhishek;Dombrowski, Stephen M.;Luciano, Mark G.
慢性脑积水（CH）的特征是存在脑室扩大、脑血流量（CBF）减少和脑组织氧输送。虽然血管内皮生长因子（VEGF）的潜在病理生理作用尚不清楚，但已知CH中的缺血缺氧事件会触发其释放。以前，我们已经发现CH后海马中VEGF受体-2（VEGFR-2）和血管密度（BVd）增加。我们研究了CH实验模型中尾状核中神经元和胶质细胞VEGFR-2密度和BVd的变化。术后2 ~ 4周（ST组）和12 ~ 16周（LT组）与手术对照组（SC组，12 ~ 16周）比较。采用免疫组化和体视学计数方法检测细胞和BVds。总的来说，百分比（%）VEGFR-2神经元在CH（ST，LT）比在SC约2倍。相比之下，胶质细胞% VEGFR-2是由10%至17%，ST和4%至11%，LT相比，在SC。血管密度显着降低，在CH比在SC在浅尾状核。脑脊液脑室容积和压力以及CBF的变化与VEGFR-2或BVd均不相关。这些观察到的结果表明，破坏力可能超过血管生成力，并可能显示VEGFR-2和BV表达之间的分离。Journal of Cerebral Blood Flow & Metabolism（2009）29，1806-1815; doi：10.1038/jcbfm.2009.98; 2009年7月15日在线发表
Chronic hydrocephalus (CH) is characterized by the presence of ventricular enlargement, decreased cerebral blood flow (CBF), and brain tissue oxygen delivery. Although the underlying pathophysiological role of vascular endothelial growth factor (VEGF) is not clear, ischemic-hypoxic events in CH are known to trigger its release. Previously, we have shown increased VEGF receptor-2 (VEGFR-2) and blood vessel density (BVd) in the hippocampus after CH. We investigated changes in neuronal and glial VEGFR-2 density and BVd in the caudate nucleus in an experimental model of CH. Animals with CH were divided into short term (ST, 2 to 4 weeks) and long term (LT, 12 to 16 weeks) and were compared with surgical controls (SCs, 12 to 16 weeks). The cellular and BVds were estimated using immunohistochemical and stereological counting methods. Overall, percentage (%) VEGFR-2 neurons were approximately two times greater in CH (ST, LT) than in SC. By comparison, glial cell % VEGFR-2 was greater by 10% to 17% in ST and 4% to 11% lower in LT compared with that in SC. Blood vessel density was significantly lower in CH than in SC in the superficial caudate. Changes in cerebrospinal fluid ventricular volume and pressure, as well as in CBF did not correlate with either VEGFR-2 or BVd. These observed findings suggest that destructive forces may outweigh angiogenic forces and possibly show a disassociation between VEGFR-2 and BV expressions. Journal of Cerebral Blood Flow & Metabolism (2009) 29, 1806-1815; doi: 10.1038/jcbfm.2009.98; published online 15 July 2009