Schistosoma mansoni:: resistant specific infection-induced gene expression in Biomphalaria glabrata identified by fluorescent-based differential display
Schistosoma mansoni:: resistant specific infection-induced gene expression in Biomphalaria glabrata identified by fluorescent-based differential display
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DOI：
10.1016/j.exppara.2004.04.004
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发表时间：
2004-05-01
影响因子：
2.1
通讯作者：
Jones, CS
中科院分区：
文献类型：
--
作者：
Lockyer, AE;Noble, LR;Jones, CS
淡水热带蜗牛Biomphalaria glabrata是曼氏血吸虫的中间宿主，曼氏血吸虫是人类肠道血吸虫病的病原体，并且菌株对寄生虫感染的易感性不同。利用新开发的基于荧光的差异显示方法，在敏感株（NHM 1742）和抗性株（NHM 1981）中同时检测了响应寄生虫感染的基因表达变化。这种RNA分析技术允许检查响应寄生虫感染的基因表达的变化，而不需要先前的序列知识，或选择可能参与蜗牛复杂的神经内分泌或防御系统的候选基因。因此，可以鉴定新的基因。最初确定了10个转录本，仅存在于来自蜗牛的耐药菌株暴露于感染时的配置文件。其中五个基因的差异表达。包括HSP 70和几种新的转录本，其中一种含有至少两个球蛋白样结构域，已被半定量RT-PCR证实。(C)2004爱思唯尔公司All rights reserved.
The freshwater tropical snail Biomphalaria glabrata is an intermediate host for Schistosoma mansoni, the causative agent of human intestinal schistosomiasis, and strains differ in their susceptibility to parasite infection. Changes in gene expression in response to parasite infection have been simultaneously examined in a susceptible strain (NHM1742) and a resistant strain (NHM1981) using a newly developed fluorescent-based differential display method. Such RNA profiling techniques allow the examination of changes in gene expression in response to parasite infection, without requiring previous sequence knowledge, or selecting candidate genes that may be involved in the complex neuroendocrine or defence systems of the snail. Thus, novel genes may be identified. Ten transcripts were initially identified, present only in the profiles derived from snails of the resistant strain when exposed to infection. The differential expression of five of these genes.. including HSP70 and several novel transcripts with one containing at least two globin-like domains, has been confirmed by semi-quantitative RT-PCR. (C) 2004 Elsevier Inc. All rights reserved.