Expression of dopamine transporter in the different cerebral regions of methamphetamine-dependent rats
Expression of dopamine transporter in the different cerebral regions of methamphetamine-dependent rats
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DOI：
10.1177/0960327114555929
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发表时间：
2015-07-01
影响因子：
2.8
通讯作者：
Yew, D. T. W.
中科院分区：
文献类型：
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作者：
Hong, S-J;Zhang, D.;Yew, D. T. W.
目的：观察多巴胺转运体（DAT）在甲基苯丙胺（MA）依赖大鼠大脑额叶皮质、伏隔核、纹状体、海马、黑质和腹侧被盖区6个区域的表达。方法：大鼠腹腔注射MA 10 mg/kg/d，连续10 d；行为变化通过条件位置偏好（CPP）测量，刻板行为（SB）分数用于确定动物成瘾。然后分别注射MA 1、2、4、8周，建立不同时期MA依赖模型。检测大鼠6个脑区DAT及DAT信使RNA的表达。结果：4个实验组的CPP、SB评分与对照组比较，差异均有统计学意义（p < 0.05）。不同实验组间比较，同一区域DAT表达以降低为主，且呈动态变化（p < 0.05）。同一实验组不同区域间比较，多个区域间DAT的表达也有显著差异（p < 0.05）。结论：在同一ma依赖时间内，6个脑区DAT的表达均以降低为主，且在同一脑区不同时间内表达有所不同。推测DAT可能在MA依赖机制中起重要作用。
Objectives: To observe the expression of the dopamine transporter (DAT) in six cerebral regions of a methamphetamine (MA)-dependent rat, which were frontal cortex, nucleus accumbens septi, striatum, hippocampus, substantia nigra and ventral tegmental area.Methods: The rats were administrated intraperitoneally with 10 mg/kg/day of MA for 10 days consecutively; the behaviour changes were measured via the conditioned place preference (CPP), and the scores of stereotyped behaviour (SB) were used to confirm animal addiction. Then, the animals were further injected with MA respectively for 1, 2, 4 and 8 weeks to establish different periods of MA-dependent models. The expressions of DAT and DAT messenger RNA in six cerebral regions were detected.Results: The results of CPP and SB scores were significant different when comparing all four experimental groups with the control group (p < 0.05). Comparing between different experimental groups, the expression of DAT mainly decreased and had dynamic changes in the same regions (p < 0.05). Comparing the different regions with each other in the same experimental group, the expression of DAT also had significant difference in several regions p < 0.05).Conclusions: The expression of DAT mainly decreased and had different in the six cerebral regions at the same MA-dependent time period as well as at different time periods in the same cerebral region. It was speculated that DAT might play a crucial role in the mechanism of MA dependence.