The value of DNA paternity identification in beef cattle: Examples from Nevada's free-range ranches
The value of DNA paternity identification in beef cattle: Examples from Nevada's free-range ranches
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DOI：
10.2527/jas.2007-0068
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发表时间：
2008-01-01
影响因子：
3.3
通讯作者：
Beattie, C. W.
中科院分区：
文献类型：
--
作者：
Gomez-Raya, L.;Priest, K.;Beattie, C. W.
以内华达肉牛为例，探讨了DNA亲子鉴定的可行性和经济价值。对来自8个牧场的2196只动物进行了15个微卫星的基因分型，总共有31,571个基因型别。计算了牧场内和牧场之间每个标记被排除的概率。还确定了15个微卫星的联合排除概率，导致任何单个牧场和跨牧场的值超过0.99。丢弃1个或2个排除概率最低的微卫星会导致联合概率大于0.99，与使用15个微卫星的概率相比，联合概率略有降低。推导了肉牛DNA亲子鉴定的效益-成本分析公式。在公牛淘汰率分别为0.20和0.30的情况下，对15个微卫星进行基因分型，每头母牛20头小牛，每投资1美元可获得1.71美元和2.44美元的收益。当1公斤小牛活重的价格与1个微卫星的基因分型成本之比大于1.1时，公牛淘汰率为0.30时，该程序就会盈利。效益-成本分析也是在不完全DNA亲子鉴定的情况下进行的，使用的DNA标记数量低于实现0.99的联合排除概率所需的DNA标记数量。在亲子鉴定不完全的情况下，使用10颗微卫星与12颗微卫星相比，效益-成本比增加了约20%。手术中的公牛数量越多，亲子鉴定计划的收益-成本比就越低。牛肉经营中的低排斥概率和高公牛数量极大地降低了效益-成本比。DNA亲子鉴定计划是可行的，并可能对自由放牧的肉牛经营有利可图。
The feasibility and economic value of DNA paternity identification were investigated and illustrated using Nevada beef cattle operations. A panel of 15 microsatellites was genotyped in 2,196 animals from 8 ranches with a total of 31,571 genotypes. Probabilities of exclusion for each marker within ranch and across ranches were computed. Joint probabilities of exclusion for the 15 microsatellites were also determined, resulting in values over 0.99 for any individual ranch and across ranches. Dropping 1 or 2 microsatellites with the lowest probabilities of exclusion resulted in joint probabilities greater than 0.99 and with marginal reduction compared with the probabilities with 15 microsatellites. Formulas for benefit-cost analysis for a DNA paternity identification program in beef cattle were derived. Genotyping 15 microsatellites with 20 calves per sire resulted in benefits of $1.71 and $2.44 per dollar invested at bull culling rates of 0.20 and 0.30, respectively. The breakpoints for the program to be profitable occurred when the ratio of the price of 1 kg of calf liveweight over the cost of genotyping 1 microsatellite was greater than 1.1 for a bull culling rate of 0.30. Benefit-cost analysis was also derived under incomplete DNA paternity identification using a lower number of DNA markers than necessary to achieve joint probabilities of exclusion of 0.99. Approximately a 20% increase in the benefit-cost ratio was achieved using 10 vs. 12 microsatellites with incomplete paternity identification. The greater the number of bulls in the operation, the lower the benefit-cost ratio of the paternity testing program. Low probabilities of exclusion and a high number of bulls in the beef operation reduced the benefit-cost ratio dramatically. The DNA paternity identification programs are feasible and may be profitable for free-range beef cattle operations.