The regulation of trefoil factor 2 expression by the transcription factor Sp3
The regulation of trefoil factor 2 expression by the transcription factor Sp3
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转录因子Sp3对三叶因子2表达的调控
DOI：
10.1016/j.bbrc.2012.09.078
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发表时间：
2012-10-19
影响因子：
3.1
通讯作者：
Ren, Jianlin
中科院分区：
文献类型：
--
作者：
Liu, Jingjing;Wang, Xu;Ren, Jianlin
三叶因子家族2（TFF2）参与粘液稳定和修复、细胞凋亡和炎症反应。先前发表的报道表明，几种生长因子和基础转录因子与TFF2的表达相关。然而，调节TFF2表达的详细机制尚未完全了解。本研究旨在评估转录因子SP3对TFF2表达的重要作用。我们首先证明了SP3和TFF2的表达水平之间存在负相关。因此，在所检查的细胞中，SP3的过表达降低了TFF2的表达水平，而SP3的抑制增加了TFF2的表达水平。此外，我们在TFF 2启动子中发现了两个GC盒，并证实了SP3与该启动子的特异性结合。总的来说，这项研究表明，Sp3是TFF2表达的主要调节因子。这些知识应该有助于我们理解SP3在调节TFF2表达中所起的作用。(C)2012 Elsevier Inc. All rights reserved.
Trefoil factor family 2 (TFF2) participates in mucus stabilization and repair, apoptosis, and inflammatory responses. Previously published reports have indicated that several growth factors and basal transcription factors are associated with the expression of TFF2. However, the detailed mechanisms that regulate TFF2 expression are not fully understood. The present study was designed to assess the essential role of the transcription factor SP3 with respect to TFF2 expression. We first demonstrated that there was a negative correlation between the expression levels of SP3 and TFF2. Thus, in the examined cells, the overexpression of SP3 decreased the expression level of TFF2, whereas the inhibition of SP3 increased the expression level of TFF2. Moreover, we discovered two GC boxes in the TFF2 promoter and confirmed the specific binding of SP3 to this promoter. On the whole, this study indicated that Sp3 was a major regulator of TFF2 expression. This knowledge should contribute to our understanding of the role that is played by SP3 in the regulation of TFF2 expression. (C) 2012 Elsevier Inc. All rights reserved.