Genetic analysis of the yeast cytoskeleton.
Genetic analysis of the yeast cytoskeleton.
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DOI：
10.1146/annurev.ge.21.120187.001355
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发表时间：
1987
影响因子：
11.1
通讯作者：
T. Huffaker;M. Hoyt;D. Botstein
中科院分区：
文献类型：
--
作者：
T. Huffaker;M. Hoyt;D. Botstein
酿酒酵母的细胞骨架和细胞周期。261有丝分裂细胞周期261偶联264指定丝状细胞骨架成分的结构基因264产生已知蛋白质功能缺陷的突变体（计划B） 264通过同源性分离已知蛋白质的基因264通过酵母细胞骨架蛋白分离基因266构建零突变267构建条件致死突变268通过突变表型鉴定基因（计划A） 270鉴定突变“影响细胞骨架功能”270相互作用基因的鉴定细胞骨架突变表型277微管蛋白突变277影响纺锤体功能的非微管蛋白突变278肌动蛋白突变280十纳米长丝突变280
INTRODUCTION 259 THE CYTOSKELETON AND CELL CYCLE OF SACCHAROMYCES CEREVISIAE. 261 Mitotic Cell Cycle " 261 Conjugation 264 Structural Genes Specifying Filamentous Cytoskeletal Components 264 GENERATING MUTANTS DEFECTIVE IN THE FUNCTIONS OF KNOWN PROTEINS (PLAN B) 264 Isolation of Genes For Known Proteins by Homology 264 Isolation of Genes via Yeast Cytoskeletal Proteins 266 Constructing Null Mutations 267 Constructing Conditional-Lethal Mutants 268 IDENTIFICATION OF GENES BY MUTANT PHENOTYPE (PLAN A) 270 Identification of Mutations "lhat Affect Cytoskeletal Function 270 Identification of Genes Whose Products Interact 273 PHENOTYPES OF CYTOSKELETAL MUTATIONS 277 Tubulin Mutants 277 Nontubulin Mutants That Affect Spindle Function 278 Actin Mutants 280 Ten-Nanometer Filament Mutants 280 CONCLUSIONS 280