Effects of calcium on nickel tolerance and accumulation in Alyssum species and cabbage grown in nutrient solution
Effects of calcium on nickel tolerance and accumulation in Alyssum species and cabbage grown in nutrient solution
复制标题
DOI：
10.1007/s11104-008-9664-7
复制
发表时间：
2008-10-01
期刊：
影响因子：
4.9
通讯作者：
Baker, Alan J. M.
中科院分区：
文献类型：
--
作者：
Chaney, Rufus L.;Chen, Kuang-Yu;Baker, Alan J. M.
利用超富集植物从富含镍的矿化和污染土壤中富集镍的植物提取镍（Ni）正处于商业化开发阶段。蛇纹石衍生土由于母质岩石的性质，土壤中Ca/Mg的比值很低。在作物植物中，土壤钙降低了镍的吸收和植物毒性，因此蛇纹石土壤的低钙可能限制了用于商业植物开采的蛇纹石土壤的超蓄积体植物耐受性。在本研究中，我们研究了蛇纹石土壤高镁特征下不同钙浓度对蛇纹石特有物种白茉莉（Alyssum murale Waldst）镍吸收和耐受性的影响。等装备。与甘蓝（Brassica oleracea L. var. capita）在营养液研究中的比较。Ca和Mg的使用水平以蛇形和正常土壤为基础，Ni的使用水平以初步试验中秋冬芽中Ni含量超过1%为基础。采用不同浓度的Ni（冬青31.6 ~ 1000 μ M，大白菜1.0 ~ 10 μ M）和Ca （0.128 ~ 5 mM）进行析因试验设计；2 mM Mg用于模拟蛇纹石土壤。苜蓿对高镍、高镁、低钙溶液的耐受性明显高于白菜。在钙镁比相对较高和较低的情况下，1000 μ M镍对茜草属植物的毒性作用较明显。在1000亩M - Ni处理下，小野田鼠地上部Ni浓度为8.18 ~ 22.8 g kg(-1)，油松田鼠地上部Ni浓度为7.60 ~ 16.0 g kg（-1）。正常溶液Ca浓度（0.8 ~ 2 mM）在所有Ni处理中均能获得最佳产量。结果表明，溶液钙水平影响地上部Ni浓度、地上部产量和根向地上部Ni转运，但两者之间的关系呈非线性关系，在Ca≤2 mM时随Ca的增加而增加，在Ca值最高时下降。结果表明，在Ca/Mg比极低的高Mg蛇形土中添加Ca可以降低Ni的植物毒性，并促进超积累植物Alyssum的年Ni提取。在植物开采中去除茎部生物量将需要钙的施用来保持充分的产量潜力。
Nickel (Ni) phytoextraction using hyperaccumulator plant species to accumulate Ni from mineralized and contaminated soils rich in Ni is undergoing commercial development. Serpentinite derived soils have a very low ratio of Ca/Mg among soils due the nature of the parent rock. In crop plants, soil Ca reduces Ni uptake and phytotoxicity, so it is possible that the low Ca of serpentine soils could limit hyperaccumulator plant tolerance of serpentine soils used for commercial phytomining. In this study, we investigated the effects of varied Ca concentration in the presence of high Mg characteristic of serpentine soils on Ni uptake and tolerance by serpentine-endemic species Alyssum murale Waldst. et Kit. and A. pintodasilvae T.R. Dudley in comparison with cabbage (Brassica oleracea L. var. capita) in a nutrient solution study. The levels of Ca and Mg used were based on serpentine and normal soils, and Ni was based on achieving over 1% Ni in Alyssum shoots in preliminary tests. Varied solution concentrations of Ni (31.6-1,000 mu M for Alyssum, 1.0-10 mu M for cabbage) and Ca (0.128-5 mM) were used in a factorial experimental design; 2 mM Mg was used to mimic serpentine soils. Alyssum spp. showed much greater tolerance to high Ni, high Mg, and low Ca solution concentrations than cabbage. For Alyssum spp., Ni induced phytotoxicity was only apparent at 1,000 mu M Ni with relatively low and high Ca/Mg quotient. In the 1,000 mu M Ni treatment, shoot Ni concentrations ranged from 8.18 to 22.8 g kg(-1) for A. murale and 7.60 to 16.0 g kg(-1) for A. pintodasilvae. Normal solution Ca concentrations (0.8-2 mM) gave the best yield across all Ni treatments for the Alyssum species tested. It was clear that solution Ca levels affected shoot Ni concentration, shoot yield and Ni translocation from root to shoot, but the relation was non-linear, increasing with increasing Ca up to 2 mM Ca, then declining at the highest Ca. Our results indicate that Ca addition to high Mg serpentine soils with very low Ca/Mg ratio may reduce Ni phytotoxicity and improve annual Ni phytoextraction by Alyssum hyperaccumulator species. Removal of shoot biomass in phytomining will require Ca application to maintain full yield potential.