The genome sequence of silkworm, Bombyx mori
The genome sequence of silkworm, Bombyx mori
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DOI：
10.1093/dnares/11.1.27
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发表时间：
2004-02-29
期刊：
影响因子：
4.1
通讯作者：
Sasaki, T
中科院分区：
文献类型：
--
作者：
Mita, K;Kasahara, M;Sasaki, T
我们对家蚕（Bombyx Mori）基因组进行了三重鸟枪测序，以获得序列草案并为全面的基因组分析建立基础资源。通过使用新开发的 RAMEN 组装器，来自全基因组鸟枪法 (WGS) 测序的序列数据被组装成 49,345 个支架，总长度为 514 Mb（包括间隙）和 387 Mb（不包括间隙）。由于蚕的基因组大小估计为 530 Mb，因此几乎 97% 的基因组已组织在支架中，其中 75% 已被测序。通过对 Bombyx EST 数据库中的 50 个特征性 Bombyx 基因和 11,202 个非冗余表达序列标签 (EST) 与 WGS 序列数据进行 BLAST 搜索，我们评估了该序列阐明大多数家蚕基因的有效性。 WGS数据分析表明，家蚕基因组包含许多重复序列，平均长度为
We performed threefold shotgun sequencing of the silkworm (Bombyx Mori) genome to obtain a draft sequence and establish a basic resource for comprehensive genome analysis. By using the newly developed RAMEN assembler, the sequence data derived from whole-genome shotgun (WGS) sequencing were assembled into 49,345 scaffolds that span a total length of 514 Mb including gaps and 387 Mb without gaps. Because the genome size of the silkworm is estimated to be 530 Mb, almost 97% of the genome has been organized in scaffolds, of which 75% has been sequenced. By carrying out a BLAST search for 50 characteristic Bombyx genes and 11,202 non-redundant expressed sequence tags (ESTs) in a Bombyx EST database against the WGS sequence data, we evaluated the validity of the sequence for elucidating the majority of silkworm genes. Analysis of the WGS data revealed that the silkworm genome contains many repetitive sequences with an average length of