Selective sensitization of bacteria to peroxide damage associated with fluoride inhibition of catalase and pseudocatalase.
Selective sensitization of bacteria to peroxide damage associated with fluoride inhibition of catalase and pseudocatalase.
复制标题
细菌对过氧化氢损伤的选择性敏感性与氟化物对过氧化氢酶和假过氧化氢酶的抑制有关。
DOI：
10.1034/j.1399-302x.2001.160105.x
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发表时间：
2001
影响因子：
--
通讯作者：
Marquis,RE
中科院分区：
文献类型：
--
作者：
Phan,TN;Kirsch,AM;Marquis,RE
氟化物和硫化物是酸性环境中已知的血红素过氧化氢酶抑制剂。发现金黄色葡萄球菌H细胞在酸性pH值3.5至4.0范围内对H2O2杀伤敏感，过氧化氢酶活性随之降低。相比之下，氟化物对 H2O2 杀灭变形链球菌 GS-5 的影响很小，该链球菌具有对氟化物不敏感的过氧化物酶活性，但对 H2O2 仍然比 isS 更敏感。在没有氟化物的情况下金黄色葡萄球菌。在很宽的 pH 范围内，氟化物（而非硫化物）对植物乳杆菌 ATCC 14431 的含锰、非血红素假过氧化氢酶也有抑制作用，这种抑制作用反映在氟化物存在下增强的 H2O2 杀灭作用。此外，我们发现过氧化氢酶呈阳性。金黄色葡萄球菌或干燥奈瑟菌可以保护过氧化氢酶阴性。 mutans 在混合悬浮液中可抵抗 H2O2 的杀伤作用，但在酸性条件下，氟化物或硫化物会损害保护作用。因此，过氧化氢酶阳性生物体可以保护过氧化氢酶阴性生物体免受过氧化物损伤，但过氧化氢酶的抑制会降低保护作用。这些发现与氟化物和过氧化物在口腔保健产品中的广泛使用有关。
Fluoride and sulfide are known inhibitors of heme catalases in acid environments.Staphylococcus aureusH cells were found to be sensitized by fluoride or sulfide to H2O2killing at acid pH values in the range of 3.5 to 4.0, and catalase activity was reduced concomitantly. In contrast, fluoride had little effect on H2O2killing ofStreptococcus mutansGS‐5, which has fluoride‐insensitive peroxidase activity, but still is more sensitive to H2O2than isS. aureusin the absence of fluoride. Fluoride but not sulfide was inhibitory also for the Mn‐containing, non‐heme pseudocatalase ofLactobacillus plantarumATCC 14431 over a wide pH range, and this inhibitory effect was reflected in enhanced H2O2killing in the presence of fluoride. In addition, we found that catalase‐positiveS. aureusorNeisseria siccacould protect catalase‐negativeS. mutansagainst killing by H2O2in mixed suspensions, but protection was compromised by fluoride or sulfide under acid conditions. Thus, catalase‐positive organisms could protect a catalase‐negative organism against peroxide damage, but inhibition of catalase reduced protection. These findings are pertinent to the widespread use of fluoride and peroxide in oral health care products.