Relationship of periodontal clinical parameters with bacterial composition in human dental plaque
Relationship of periodontal clinical parameters with bacterial composition in human dental plaque
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DOI：
10.1007/s00203-013-0883-9
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发表时间：
2013-06-01
影响因子：
2.8
通讯作者：
Yamashita, Yoshihisa
中科院分区：
文献类型：
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作者：
Fujinaka, Hidetake;Takeshita, Toru;Yamashita, Yoshihisa
在口腔中已鉴定出600多种细菌，但只有有限数量的细菌与牙周炎有很强的相关性。本研究的目的是提供与牙周状况相关的牙菌斑中微生物群的全面概述。从90名受试者的牙菌斑取样，并使用16 S rRNA基因的末端限制性片段长度多态性基于细菌组成对受试者进行聚类。在这里，我们评估了（1）集群之间的牙周临床参数;（2）龈下细菌组成与龈上细菌组成的相关性;（3）使用图形高斯模型分析牙菌斑中细菌种间的相关性。具有龈下菌斑中致病菌高患病率的聚类1（C1）显示参数值增加。非致病性细菌高患病率的聚类2a（C2 a）中的参数值明显低于C1中的参数值。龈上菌斑中非致病性细菌患病率低的群集显示参数值增加。龈下菌斑与龈上菌斑的菌型有显著相关性。主要病原菌如牙龈卟啉单胞菌在C1中与其他致病菌形成了一个网络，而非致病菌如罗氏菌和劳氏菌，倾向于与C2 a中的致病物种网络竞争。牙周炎状态与非致病性菌种以及致病性菌种有关，表明细菌种间连接影响牙菌斑毒力。
More than 600 bacterial species have been identified in the oral cavity, but only a limited number of species show a strong association with periodontitis. The purpose of the present study was to provide a comprehensive outline of the microbiota in dental plaque related to periodontal status. Dental plaque from 90 subjects was sampled, and the subjects were clustered based on bacterial composition using the terminal restriction fragment length polymorphism of 16S rRNA genes. Here, we evaluated (1) periodontal clinical parameters between clusters; (2) the correlation of subgingival bacterial composition with supragingival bacterial composition; and (3) the association between bacterial interspecies in dental plaque using a graphical Gaussian model. Cluster 1 (C1) having high prevalence of pathogenic bacteria in subgingival plaque showed increasing values of the parameters. The values of the parameters in Cluster 2a (C2a) having high prevalence of non-pathogenic bacteria were markedly lower than those in C1. A cluster having low prevalence of non-pathogenic bacteria in supragingival plaque showed increasing values of the parameters. The bacterial patterns between subgingival plaque and supragingival plaque were significantly correlated. Chief pathogens, such as Porphyromonas gingivalis, formed a network with other pathogenic species in C1, whereas a network of non-pathogenic species, such as Rothia sp. and Lautropia sp., tended to compete with a network of pathogenic species in C2a. Periodontal status relates to non-pathogenic species as well as to pathogenic species, suggesting that the bacterial interspecies connection affects dental plaque virulence.