Screening of Rice Varieties for Endosperm Storage Proteins
Screening of Rice Varieties for Endosperm Storage Proteins
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胚乳储存蛋白水稻品种的筛选
DOI：
10.1111/j.1439-0523.1990.tb00461.x
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发表时间：
1990
期刊：
影响因子：
2
通讯作者：
T. Kumamaru
中科院分区：
文献类型：
--
作者：
A. Bhowmik;T. Omura;T. Kumamaru
以118个水稻品种（系）为材料，进行了遗传多样性分析。来自孟加拉国的一个品种的胚乳贮藏蛋白进行了分析。以粳稻标准品种“金麻泽”为材料，通过SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）图谱的观察进行筛选。根据多肽带型分为6类。对所选品种进行双向凝胶电泳，以确保SDS-PAGE结果。对提取的蛋白质组分的分析表明，一个品种的醇溶蛋白含量低，一个品种的谷蛋白含量高。
A total of 118 rice varieties/lines (Oryza sativa L.) from Bangladesh were analyzed for endosperm storage proteins on a single seed basis. The screening was done by observing the profiles of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) with standard japonica variety ‘Kinmaze’. Six groups were classified on the basis of the poly-peptide banding patterns. Two-dimensional gel electrophoresis was performed to ensure the SDS-PAGE results in selected varieties. The analysis of extracted protein fractions revealed that one variety had low prolamin and one variety had high glutelin content.