Characterization of an influenza A virus isolated from pigs during an outbreak of respiratory disease in swine and people during a county fair in the United States
Characterization of an influenza A virus isolated from pigs during an outbreak of respiratory disease in swine and people during a county fair in the United States
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DOI：
10.1016/j.vetmic.2009.01.003
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发表时间：
2009-05-28
影响因子：
3.3
通讯作者：
Zhang, Yan
中科院分区：
文献类型：
--
作者：
Vincent, Amy L.;Swenson, Sabrina L.;Zhang, Yan
2007年8月，猪和人在参加俄亥俄州的一个县集市时被临床感染了一种流感样疾病。从猪和人身上鉴定出甲型流感病毒，分离的病毒特征为猪H1N1，与目前在美国猪群中流行的猪病毒相似。本文报道了猪分离株A/SW/OH/511445/2007 （OH07）的感染和传播实验研究。结果表明，OH07病毒在猪中具有致病性，可在猪间传播，且不能与多种猪HI抗血清发生交叉反应。自然暴露的猪在与实验感染的猪接触后最早3天，最长7天就会传播病毒。这表明在博览会上处理猪的人有机会被曝光。对OH07分离株的分子分析表明，这8个基因片段与目前流行的三重配猪流感病毒相似。然而，与同一基因簇的当代猪病毒相比，在HA基因和整个基因组中证实了许多核苷酸变化导致氨基酸变化。目前尚不清楚是否有任何氨基酸变化与这种病毒感染人类的能力有关。根据猪实验模型中OH07病毒的特征以及记录在案的人类传播情况，有必要密切监测该病毒在猪和人群中的传播情况。Elsevier B.V.出版
In August 2007, pigs and people became clinically affected by an influenza-like illness during attendance at an Ohio county fair. Influenza A virus was identified from pigs and people, and the virus isolates were characterized as swine H1N1 similar to swine viruses currently circulating in the U.S. pig population. The swine isolate, A/SW/OH/511445/2007 (OH07), was evaluated in an experimental challenge and transmission study reported here. Our results indicate that the OH07 virus was pathogenic in pigs, was transmissible among pigs, and failed to cross-react with many swine HI anti-sera. Naturally exposed pigs shed virus as early as 3 days and as long as 7 days after contact with experimentally infected pigs. This suggests there was opportunity for exposure of people handling the pigs at the fair. The molecular analysis of the OH07 isolates demonstrated that the eight gene segments were similar to those of currently circulating triple reassortant swine influenza viruses. However, numerous nucleotide changes leading to amino acid changes were demonstrated in the HA gene and throughout the genome as compared to contemporary swine viruses in the same genetic cluster. It remains unknown if any of the amino acid changes were related to the ability of this virus to infect people. The characteristics of the OH07 virus in our pig experimental model as well as the documented human transmission warrant close monitoring of the spread of this virus in pig and human populations. Published by Elsevier B.V.