Mageeibacillus indolicus gen. nov., sp. nov.: a novel bacterium isolated from the female genital tract.
Mageeibacillus indolicus gen. nov., sp. nov.: a novel bacterium isolated from the female genital tract.
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DOI：
10.1016/j.anaerobe.2014.12.003
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发表时间：
2015-04
期刊：
影响因子：
2.3
通讯作者：
Hillier SL
中科院分区：
文献类型：
--
作者：
Austin MN;Rabe LK;Srinivasan S;Fredricks DN;Wiesenfeld HC;Hillier SL
对用常规培养方法从人子宫内膜中分离到的3株细菌进行了生化鉴定，并进行了16S rRNA基因测序和系统发育分析。分离的菌株为不能运动、专性厌氧、无芽胞形成、无糖解、非纤维素化、吲哚阳性、革兰氏阳性杆菌。细胞壁脂肪酸组成分析表明，C14：0、C16：0、C18：2、ω6、9c、C18：1、ω9c和C18：0是主要的脂肪酸组成。测得DNA摩尔%G+C为44.2%。16S rRNA基因序列分析表明，与最接近的培养细菌菌株Saccharofermentans acetigene的序列相似性仅为91%。根据基因和表型数据，这三个分离物被认为是同一物种的成员，数据表明它代表了梭状芽胞杆菌目的一个新属和新种，与反刍球菌科中的Clostridium rRNA簇III有关联。我们建议将其命名为Mageeibacillusindolicus gen。Nov.，sp.11月模式菌株为BAA-2120T和CCUG 59143T。
Three isolates of a bacterium recovered from human endometrium using conventional culture methods were characterized biochemically and subjected to 16S rRNA gene sequencing and phylogenetic analysis. Isolates were non-motile, obligately anaerobic, non-spore forming, asaccharolytic, non-cellulolytic, indole positive, Gram positive rods. Cell wall fatty acid profiling revealed C14:0, C16:0, C18:2 ω6, 9c, C18:1 ω9c and C18:0 to be the major fatty acid composition. The DNA mol % G+C was determined to be 44.2%. 16S rRNA gene sequence analysis revealed only 91% sequence similarity with the closest cultivated bacterial isolate, Saccharofermentans acetigenes. Based on genotypic and phenotypic data, all three isolates are considered to be members of the same species and data suggest it represents a novel genus and species in the order Clostridiales with an association with Clostridium rRNA cluster III within the family Ruminococcaceae. We propose the name, Mageeibacillus indolicus gen. nov., sp. nov. The type strain is BAA-2120T and CCUG 59143T.