Colonization and Nitrogen-Fixing Ability of Herbaspirillum sp. Strain B501 gfp1 and Assessment of Its Growth-Promoting Ability in Cultivated Rice
Colonization and Nitrogen-Fixing Ability of Herbaspirillum sp. Strain B501 gfp1 and Assessment of Its Growth-Promoting Ability in Cultivated Rice
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DOI：
10.1264/jsme2.22.197
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发表时间：
2007-09
影响因子：
2.2
通讯作者：
M. Zakria;J. Njoloma;Y. Saeki;S. Akao
中科院分区：
文献类型：
--
作者：
M. Zakria;J. Njoloma;Y. Saeki;S. Akao
研究了从野生稻中分离的固氮内生菌Herbaspirillum sp.菌株B501 gfp 1在102和108 cells ml-1浓度下感染栽培稻Oryza sativa cv.日本晴两种剂量均导致根和茎（基部茎和叶鞘）的定殖。未观察到叶的定殖。根中的细菌数量高于茎。细菌在根表皮细胞间隙和侧根连接处的间隙中定殖。它们还定殖于基茎的表皮和周鞘以及叶鞘真皮组织系统的亚表皮组织。定殖细菌将大量的15 N2掺入受感染的植物中。接种植物的干重和鲜重也高于对照（未接种）植物。
The endophytic colonization, nitrogen fixation, and plant growth-promoting abilities of Herbaspirillum sp. strain B501 gfp1, which is a diazotrophic endophyte isolated from wild rice, were studied after infection (at 102 and 108 cells ml-1) of seedlings of cultivated rice Oryza sativa cv. Nipponbare. Both doses resulted in colonization of the roots and stem (basal stem and leaf sheath). No colonization of leaves was observed. Higher bacterial populations were observed in the roots than stems. The bacteria colonized the intercellular spaces of the root epidermis and the spaces at the junctions of the lateral roots. They also colonized the epidermis and pericycle of the basal stem and the sub-epidermal tissues of the dermal tissue system of the leaf sheath at later stages. The colonizing bacteria incorporated significant amounts of 15N2 into the infected plants. The inoculated plants also had higher dry weights and fresh weights than the control (uninoculated) plants.