Radial caseinolysis in agarose: a simple method for detection of plasminogen activator in the presence of inhibitory substances and serum.
Radial caseinolysis in agarose: a simple method for detection of plasminogen activator in the presence of inhibitory substances and serum.
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DOI：
10.1016/0003-2697(81)90564-9
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发表时间：
1981-03
影响因子：
2.9
通讯作者：
O. Saksela
中科院分区：
文献类型：
--
作者：
O. Saksela
A technically easy, sensitive, and reproducible method was developed for detection of plasminogen activator in cell growth media and cell extracts. Samples to be assayed were applied into wells cut in opaque casein-containing agarose gels with and without plasminogen. Diameters of the clear circular area resulting from proteolysis around the wells were proportional to the amount of applied plasminogen activator. Sodium dodecyl sulphate (NaDodSO4) was added to the samples in order to inactivate the protease-inhibiting substances present. NaDodSO4denatured these protease inhibitors irreversibly while its inhibitory effect on plasminogen activator and plasmin was reversible and was diluted out during radial diffusion of the samples. These properties make the assay superior to the conventional125I-fibrin method for several purposes.
