Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant
Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant
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DOI：
10.1371/journal.pone.0085998
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发表时间：
2014-02-11
期刊：
影响因子：
3.7
通讯作者：
Davies, Angharad P.
中科院分区：
文献类型：
--
作者：
Pascoe, Ben;Dams, Lucy;Davies, Angharad P.
We describe the first in vitro model of dormancy in Staphylococcus aureus, showing that cells are generated which can be resuscitated by addition of spent medium supernatant taken from cultures of the same organism. Over 30 days, culturable counts in dormant cultures of S. aureus SH1000 fell from 10(6)-10(7) cfu/ml to 600-fold increase in bacterial growth. Resuscitation was a specific effect, greatly reduced by boiling or addition of trypsin to the spent supernatant. Supernatant also effected a reduction in lag phase of dormant cultures. SEM demonstrated the presence of small coccoid cells in dormant cultures. The results are similar to those seen with resuscitation promoting factors (Rpfs) in actinobacteria. This is the first time resuscitation has been demonstrated in Staphylococcus aureus, which is an important human pathogen. A better understanding of control and reactivation of dormant cells could lead to major improvements in managing staphylococcal infections; resuscitation could be an important step in restoring susceptibility to antibiotic treatment.
