Role of membrane mimetics on biophysical EPR studies of membrane proteins
Role of membrane mimetics on biophysical EPR studies of membrane proteins
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DOI：
10.1016/j.bbamem.2023.184138
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发表时间：
2023-02-11
影响因子：
3.4
通讯作者：
Lorigan，Gary A.
中科院分区：
文献类型：
--
作者：
Sahu，Indra D.;Lorigan，Gary A.
生物膜对于提供膜蛋白在功能状态下的稳定性至关重要。膜蛋白的生物物理电子顺磁共振 (EPR) 研究需要功能稳定的均质样品，以获得蛋白质的相关结构动力学。生物物理 EPR 测量所需的合适膜环境的优化已取得重大进展。然而，没有通用的膜模拟系统可以溶解所有适合生物物理 EPR 研究的膜蛋白，同时保持功能完整性。需要付出很大的努力来优化样品条件，以获得更好的膜蛋白 EPR 数据质量，从而提供有关结构动力学的有意义的信息。在这篇简短的评论中，我们将讨论生物物理 EPR 测量的膜模拟的重要方面以及最近的一些例子的当前进展。
Biological membranes are essential in providing the stability of membrane proteins in a functional state. Functionally stable homogeneous sample is required for biophysical electron paramagnetic resonance (EPR) studies of membrane proteins for obtaining pertinent structural dynamics of the protein. Significant progresses have been made for the optimization of the suitable membrane environments required for biophysical EPR measurements. However, no universal membrane mimetic system is available that can solubilize all membrane proteins suitable for biophysical EPR studies while maintaining the functional integrity. Great efforts are needed to optimize the sample condition to obtain better EPR data quality of membrane proteins that can provide meaningful information on structural dynamics. In this mini-review, we will discuss important aspects of membrane mimetics for biophysical EPR measurements and current progress with some of the recent examples.