Overexpression of a cotton nonspecific lipid transfer protein gene, GhLTP4, enhances drought tolerance by remodeling lipid profiles, regulating abscisic acid homeostasis and improving tricarboxylic acid cycle in cotton
Overexpression of a cotton nonspecific lipid transfer protein gene, GhLTP4, enhances drought tolerance by remodeling lipid profiles, regulating abscisic acid homeostasis and improving tricarboxylic acid cycle in cotton
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DOI：
10.1016/j.envexpbot.2022.104991
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发表时间：
2022-07
影响因子：
5.7
通讯作者：
Dayong Zhang;Jining Li;Miaomiao Li;Zimeng Cheng;Qian Xu;Xiaohui Song;Xiaoguang Shang;Wangzhen Guo
中科院分区：
文献类型：
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作者：
Dayong Zhang;Jining Li;Miaomiao Li;Zimeng Cheng;Qian Xu;Xiaohui Song;Xiaoguang Shang;Wangzhen Guo
干旱是世界范围内造成农作物严重减产的主要非生物胁迫。脂质转运蛋白（LTP）在植物生长和逆境胁迫信号转导过程中起着重要作用。然而，干旱胁迫所涉及的调节回路在很大程度上仍然没有得到解决。本文报道了一种非特异性的脂质转运蛋白GhLTP4（synonymal Pfs6，GenBank：ABO42261）在棉花纤维细胞中优先表达，并受植物激素脱落酸（阿坝）和干旱胁迫的强烈诱导。与野生型相比，GhLTP4基因的过表达和下调分别导致棉花耐旱性的提高和降低。在正常条件下，过量表达GhLTP4显著增加了转基因棉花叶片中的脂类成分和蜡质的积累。在干旱胁迫下，转GhLTP4基因棉花表现出脂质结构的改变，阿坝含量增加，三羧酸循环过程改善。本研究揭示了GhLTP4基因在棉花耐旱性调控中的功能和机制，为棉花耐旱育种提供了有价值的遗传材料。
Drought is a major abiotic stress that causes severely crop yield loss worldwide. Lipid transfer proteins (LTPs) play an essential role during plant growth and cell signaling transduction associated with stress responses. However, the regulatory circuits involved in drought stress remain largely unresolved. Here, we reported that a nonspecific lipid transfer protein, namelyGhLTP4, positively modulated drought stress tolerance in cotton.GhLTP4(synonymPfs6, GenBank: ABO42261) was preferentially expressed in fiber cells, while, it was also strongly induced by phytohormone abscisic acid (ABA) and drought stress treatments. Compared with wild type, over- and down-expression ofGhLTP4in cotton led to the increased and decreased drought tolerance, respectively. Overexpression ofGhLTP4remarkably increased lipid components and the accumulation of culticular waxes in transgenic cotton leaves under normal condition. Under drought stress,GhLTP4-overexpressing transgenic cotton exhibited the remodeled lipid profiles, more ABA contents, and the improved tricarboxylic acid cycle process. Taken together, we reveal the function and underlying mechanisms ofGhLTP4in regulating cotton drought tolerance and create valuable genetic accessions for cotton drought-tolerant breeding.