DETECTION OF GANGLIOSIDES THAT BIND CHOLERA-TOXIN - DIRECT BINDING OF I-125 LABELED TOXIN TO THIN-LAYER CHROMATOGRAMS
DETECTION OF GANGLIOSIDES THAT BIND CHOLERA-TOXIN - DIRECT BINDING OF I-125 LABELED TOXIN TO THIN-LAYER CHROMATOGRAMS
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DOI：
10.1016/0003-2697(80)90667-3
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发表时间：
1980-01-01
影响因子：
2.9
通讯作者：
GINSBURG, V
中科院分区：
文献类型：
--
作者：
MAGNANI, JL;SMITH, DF;GINSBURG, V
结合霍乱毒素的神经节苷可以通过将125i标记的毒素直接与薄层色谱结合，然后进行放射自显影来检测。该方法灵敏度高，在细胞总脂提取物的色谱图上可检测到0.1 ng （70 f[飞托]mol）的GMI。该方法可能有助于确定其他毒素或蛋白质的受体，如某些凝集素、抗体和结合碳水化合物的激素。
Gangliosides that bind cholera toxin can be detected by the direct binding of 125I-labeled toxin to thin-layer chromatograms followed by autoradiography. The method is very sensitive: 0.1 ng of GMI (70 f[femto]mol) can be detected on chromatograms of total lipid extracts of cells. The method may be useful in defining the receptors of other toxins or proteins such as some lectins, antibodies and hormones that bind carbohydrates.