Expression profile of MTA1 in adult mouse tissues
Expression profile of MTA1 in adult mouse tissues
复制标题
MTA1 在成年小鼠组织中的表达谱。
DOI：
10.1016/j.tice.2009.04.002
复制
发表时间：
2009-12-01
期刊：
影响因子：
2.6
通讯作者：
Zhang, Yuanqiang
中科院分区：
文献类型：
--
作者：
Li, Wei;Ma, Li;Zhang, Yuanqiang
MTA1作为核小体重塑和去乙酰化复合体(NuRD)的组成部分，被认为通过影响染色质重塑状态来调节转录。尽管MTA1与几种肿瘤细胞系和组织的转移潜能密切相关，但它也可以通过改变关键靶基因的乙酰化状态来调节不同的细胞通路。然而，它的基本生理功能还没有被表征。为了进一步研究该蛋白在哺乳动物中可能的生理作用，作者利用半定量RT-PCR、Western blotting和免疫组织化学等技术组合，研究了小鼠MTA1在各种成年小鼠组织中的表达模式。在神经系统、心血管系统、呼吸系统、消化系统、免疫系统、内分泌系统、泌尿系统、生殖系统和感觉器官系统的多种组织/细胞中观察到阳性信号。MTA1定位于胞浆和胞核，并在胞核内聚集。在成熟小鼠中，MTA1表达在不断进行增殖或自我更新的细胞类型中，如睾丸，以及不经常进行增殖或自我更新的细胞类型，如脑、肝脏和肾脏。这种差异表达表明，这种蛋白质在小鼠器官中发挥着不同的功能。(C)2009爱思唯尔有限公司。保留所有权利。
MTA1, as a constituent of the nucleosome-remodeling and -deacetylation complex (NuRD), is thought to modulate transcription by influencing the status of chromatin remodeling. Despite its strong correlation with the metastatic potential of several cancer cell lines and tissues, MTA1 can also regulate divergent cellular pathways by modifying the acetylation status of crucial target genes. However, its fundamental physiological functions have not been characterized. To further address the possible physiological role of this protein in mammals, the authors examined the expression pattern of mouse MTA1 in a variety of adult mouse tissues by a combination of techniques, including semi-quantitative RT-PCR, Western blotting and immunohistochemistry. Positive signals were observed on variety of tissues/cells in multiple systems including nervous, cardiovascular, respiratory, digestive, immune, endocrine, urinary, reproductive and sensory organ systems. MTA1 was localized in both the cytoplasm and the nuclei, and was accumulated in the nuclei. In mature mice, MTA1 expression was seen in cell types that constantly undergo proliferation or self-renewal, such as testis and cell types not constantly engaged in proliferation or self-renewal, such as brain, liver and kidney. This differential expression suggests that this protein serves distinct functions in murine organs. (C) 2009 Elsevier Ltd. All rights reserved.