A SENSITIVE AND RELIABLE LOCOMOTOR RATING-SCALE FOR OPEN-FIELD TESTING IN RATS
A SENSITIVE AND RELIABLE LOCOMOTOR RATING-SCALE FOR OPEN-FIELD TESTING IN RATS
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DOI：
10.1089/neu.1995.12.1
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发表时间：
1995-02-01
影响因子：
4.2
通讯作者：
BRESNAHAN, JC
中科院分区：
文献类型：
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作者：
BASSO, DM;BEATTIE, MS;BRESNAHAN, JC
脊髓挫伤后的行为评估长期以来一直关注开放领域的运动，使用Tarlov和Klinger（1954）开发的评级量表的修改。然而，几个小组正在进行的修改，使实验室间比较运动结果的措施困难。本研究的目的是开发一个有效的，扩展的，明确的运动评定量表，标准化运动结果的措施，在实验室。成年大鼠（n = 85）在T7-9脊髓水平与电磁或重量下降装置挫伤。在受伤前、术后第一天或第二天以及之后长达10周的时间内对运动行为进行评估。根据观察到的运动恢复模式序列，确定评分类别和属性，并进行操作定义和排名。这些类别形成了Basso，Beattie，Bresnahan（BBB）运动评定量表。数据表明，BBB量表是一种有效的和预测性的运动恢复措施，能够区分由于不同的伤害行为的结果，并预测病变中心的解剖学改变。评分者间信度测试表明，具有广泛不同的行为测试经验的考官可以一致地应用该量表并获得相似的分数。BBB运动评定量表为研究者提供了一个更具鉴别力的行为结果测量方法，以评估脊髓损伤后的治疗。
Behavioral assessment after spinal cord contusion has long focused on open field locomotion using modifications of a rating scale developed by Tarlov and Klinger (1954). However, on-going modifications by several groups have made interlaboratory comparison of locomotor outcome measures difficult. The purpose of the present study was to develop an efficient, expanded, and unambiguous locomotor rating scale to standardize locomotor outcome measures across laboratories. Adult rats (n = 85) were contused at T7-9 cord level with an electromagnetic or weight drop device. Locomotor behavior was evaluated before injury, on the first or second postoperative day, and then for up to 10 weeks. Scoring categories and attributes were identified, operationally defined, and ranked based on the observed sequence of locomotor recovery patterns. These categories formed the Basso, Beattie, Bresnahan (BBB) Locomotor Rating Scale. The data indicate that the BBB scale is a valid and predictive measure of locomotor recovery able to distinguish behavioral outcomes due to different injuries and to predict anatomical alterations at the lesion center. Interrater reliability tests indicate that examiners with widely varying behavioral testing experience can apply the scale consistently and obtain similar scores. The BBB Locomotor Rating Scale offers investigators a more discriminating measure of behavioral outcome to evaluate treatments after spinal cord injury.