Lactobacillus salivarius CECT 5713, a potential probiotic strain isolated from infant feces and breast milk of a mother-child pair
Lactobacillus salivarius CECT 5713, a potential probiotic strain isolated from infant feces and breast milk of a mother-child pair
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DOI：
10.1016/j.ijfoodmicro.2006.06.011
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发表时间：
2006-10-15
影响因子：
5.4
通讯作者：
Rodriguez, J. M.
中科院分区：
文献类型：
--
作者：
Martin, R.;Jimenez, E.;Rodriguez, J. M.
在这项研究中，唾液乳杆菌CECT 5713最初是从一个月大的母乳喂养婴儿的粪便中分离出来的。由于有人认为母乳喂养的婴儿的肠道微生物区系反映了母亲母乳的微生物区系，我们调查了这一特定菌株是否存在于各自母亲的母乳中。RAPD和PFGE分析表明，该生物液中存在唾液乳杆菌CECT 5713菌株。据我们所知，这是从母乳中分离出的唾液乳杆菌菌株的首次报道。唾液乳杆菌CECT5713产生L乳酸盐、醋酸盐和过氧化氢，这可能是其对本研究中使用的大多数指示生物具有抑菌活性的原因；此外，该菌株在模拟胃肠道条件下显示出高存活率。最后，它与Caco-2细胞有很强的粘附性，在体外不能产生生物胺，不能降解胃粘液。(C)2006爱思唯尔B.V.保留所有权利。
in this study, Lactobacillus salivarius CECT 5713 was originally isolated from feces of a one-month-old breast-fed infant. Since it has been suggested that the gut microbiota of breast-fed infants reflects that of the maternal breast milk, we investigated if this specific strain was present in breast milk of the respective mother. RAPD and PFGE analysis revealed the presence of the strain L. salivarius CECT 5713 in this biological fluid. To our knowledge, this is the first report of a L. salivarius strain isolated from breast milk. L. salivarius CECT 5713 produced L-lactate, acetate and hydrogen peroxide, which may be responsible for its antimicrobial activity against most of the indicator organisms used in this study; in addition, this strain showed a high survival rate after exposition to conditions simulating those found in the gastrointestinal tract. Finally, it was strongly adhesive to Caco-2 and HT-29 cells did not produce biogenic amines and were unable to degrade gastric mucin in vitro. (c) 2006 Elsevier B.V. All rights reserved.