Expression of glycerol 3‐phosphate dehydrogenase gene (CvGPD1) in salt‐tolerant yeast Candida versatilis is stimulated by high concentrations of NaCl
Expression of glycerol 3‐phosphate dehydrogenase gene (CvGPD1) in salt‐tolerant yeast Candida versatilis is stimulated by high concentrations of NaCl
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DOI：
10.1002/yea.1550
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发表时间：
2008-02
期刊：
影响因子：
2.6
通讯作者：
Yasuo Watanabe;K. Nagayama;Y. Tamai
中科院分区：
文献类型：
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作者：
Yasuo Watanabe;K. Nagayama;Y. Tamai
我们从耐盐酵母通用念珠菌中克隆了 3-磷酸甘油脱氢酶 (GPDH) 基因 (CvGPD1)。当CvGPD1在甘油合成缺陷的酿酒酵母细胞中表达时，重组菌株的耐盐性增强，并且证实了NADP+依赖的GPDH（EC 1.1.1.94）、Cvgpd1p合成和甘油合成的恢复。高浓度的 NaCl 会刺激 C. versatilis 细胞中 CvGPD1 的转录。还讨论了 CvGPD1 的表达与日本调味料（味噌和酱油醪）糊状物中 C. versatilis 细胞生长之间的关系。测序的DNA片段的登录号是AB296385。版权所有 © 2007 约翰·威利父子有限公司
We cloned the glycerol 3‐phosphate dehydrogenase (GPDH) gene (CvGPD1) from salt‐tolerant yeast Candida versatilis. When CvGPD1 was expressed in glycerol synthesis‐deficient Saccharomyces cerevisiae cells, the salt tolerance of the recombinant strain was enhanced, and NADP+‐dependent GPDH (EC 1.1.1.94), Cvgpd1p synthesis and recovery of glycerol synthesis were confirmed. The transcription of CvGPD1 in C. versatilis cells was stimulated by high concentrations of NaCl. The relationship between expression of CvGPD1 and growth of C. versatilis cells in the mash of Japanese seasonings (miso‐ and shoyu‐moromi) is also discussed. The Accession No. for the sequenced DNA fragment is AB296385. Copyright © 2007 John Wiley & Sons, Ltd.