Characterization of GATA-1+ hemangioblastic cells in the mouse embryo
Characterization of GATA-1+ hemangioblastic cells in the mouse embryo
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DOI：
10.1038/sj.emboj.7601480
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发表时间：
2007-01-10
期刊：
影响因子：
11.4
通讯作者：
Yamamoto, Masayuki
中科院分区：
文献类型：
--
作者：
Yokomizo, Tomomasa;Takahashi, Satoru;Yamamoto, Masayuki
成血管细胞被认为是造血祖细胞的来源之一，但对其在小鼠胚胎中的定位和命运知之甚少。我们在此表明，在胚胎第 7.5 天，共表达造血标记物 GATA-1 和内皮标记物 VE-钙粘蛋白的细胞子集定位于卵黄囊血岛。克隆分析表明，从 E7.0-7.5 胚胎中分离的 GATA-1(+) 细胞包含造血细胞和内皮细胞的共同前体细胞。此外，该前体具有原始且确定的造血双潜能。通过使用转基因互补拯救方法，GATA-1(+)细胞来源的祖细胞在Runx1缺陷小鼠中被选择性恢复。在获救的小鼠中，恢复了明确的红细胞生成，但获救的祖细胞没有表现出多谱系造血或主动脉内造血簇。这些结果提供了胚胎外区域存在GATA-1(+)成血管细胞及其对胚胎造血功能的功能贡献的证据。
Hemangioblasts are thought to be one of the sources of hematopoietic progenitors, yet little is known about their localization and fate in the mouse embryo. We show here that a subset of cells co-expressing the hematopoietic marker GATA-1 and the endothelial marker VE-cadherin localize on the yolk sac blood islands at embryonic day 7.5. Clonal analysis demonstrated that GATA-1(+) cells isolated from E7.0-7.5 embryos include a common precursor for hematopoietic and endothelial cells. Moreover, this precursor possesses primitive and definitive hematopoietic bipotential. By using a transgenic complementation rescue approach, GATA-1(+) cell-derived progenitors were selectively restored in Runx1-deficient mice. In the rescued mice, definitive erythropoiesis was recovered but the rescued progenitors did not display multilineage hematopoiesis or intra-aortic hematopoietic clusters. These results provide evidence of the presence of GATA-1(+) hemangioblastic cells in the extra-embryonic region and also their functional contribution to hematopoiesis in the embryo.