Molecular epizootiology of avian infectious bronchitis in Russia
Molecular epizootiology of avian infectious bronchitis in Russia
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DOI：
10.1080/03079450600921008
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发表时间：
2006-10-01
期刊：
影响因子：
2.8
通讯作者：
Drygin, Vladimir V.
中科院分区：
文献类型：
--
作者：
Bochkov, Yury A.;Batchenko, Galina V.;Drygin, Vladimir V.
对1998年至2002年从俄罗斯鸡群中分离的传染性支气管炎病毒（IBV）进行了分子鉴定。使用对应于病毒基因组中最保守的3 '-非翻译区和最可变的S1基因区的两组引物，通过逆转录酶-聚合酶链反应检测了250多份田间样品。91个IBV分离株的特征在于由136至558个核苷酸的S1基因高变区的系统发育分析。主要分离株组（38种病毒）与自20世纪70年代初以来在俄罗斯流行的马萨诸塞州基因型菌株显示出非常密切的序列关系。其他22个俄罗斯IBV的分析区域与欧洲基因型毒株相似（89 - 98%同一性），包括D274（9个分离株）、793/B（10个分离株）、B1648、624/I和意大利-02（每组1个分离株）。两个分离物从非常遥远的地理位置在俄罗斯（远东和欧洲部分）聚集在一起，与中国株的QXIBV基因型。其余27个俄罗斯分离株均未显示与序列数据库中已知IBV毒株的密切序列关系。这些变异病毒中的大多数聚集在六种新的俄罗斯基因型中，通常与它们的地理位置相关。剩下的五个被放在这些独特的群体之外，也代表了新的基因型。这些数据首次证明了在俄罗斯传播的IBV分离株的高度遗传多样性。
Molecular characterization of infectious bronchitis viruses ( IBVs) isolated between 1998 and 2002 from chickens in Russia was performed. More than 250 field samples were tested by reverse transcriptase-polymerase chain reaction using two sets of primers corresponding to the most conserved 3'-untranslated region and the most variable S1 gene region of the viral genome. Ninety-one IBV isolates were characterized by phylogenetic analysis of the S1 gene hypervariable region comprising 136 to 558 nucleotides. The major group of isolates ( 38 viruses) showed very close sequence relationship with strains of the Massachusetts genotype circulating in Russia since the early 1970s. The analysed region of the other 22 Russian IBVs was similar ( from 89 to 98% identity) to that from the strains of European genotypes including D274 ( nine isolates), 793/B ( 10 isolates), and B1648, 624/I and Italy-02 ( one isolate in each group). Two isolates from very distant geographic locations in Russia ( Far East and the European part) clustered together with Chinese strains of QXIBV genotype. None of the remaining 27 Russian isolates showed a close sequence relationship with known IBV strains available in sequence databases. The majority of these variant viruses clustered into the six novel Russian genotypes, often correlating with their geographic location. The remaining five of them were placed outside these unique groups, also representing new genotypes. These data for the first time demonstrated the high genetic diversity of IBV isolates circulating in Russia.