Amantadine-resistance among H5N1 avian influenza viruses isolated in Northern China
Amantadine-resistance among H5N1 avian influenza viruses isolated in Northern China
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中国北方分离的H5N1禽流感病毒对金刚烷胺的耐药性
DOI：
10.1016/j.antiviral.2007.08.007
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发表时间：
2008-01-01
影响因子：
7.6
通讯作者：
Tian, Yong
中科院分区：
文献类型：
--
作者：
He, Guimei;Qiao, Jian;Tian, Yong
对2001 ~ 2005年北方省鸡源H5 N1亚型禽流感病毒进行了金刚烷胺耐药性检测，并对该地区H5 N1亚型禽流感病毒金刚烷胺使用情况进行了调查。MDCK细胞鼠疫还原试验表明，83.3%（5/6）的菌株为金刚烷胺耐药菌株。M2序列分析显示，5株耐药菌株中有4株在31位含有可赋予金刚烷胺耐药的点突变（Ser至Asn）。这些结果表明，在中国北方分离的金刚烷胺耐药病毒的发生率特别高。在对金刚烷胺使用情况的调查中发现，该地区养禽场金刚烷胺的使用较为广泛，这可能是造成该地区养禽场金刚烷胺耐药发生率较高的原因之一。(C)2007 Elsevier B. V.保留所有权利。
We tested the amantadine-resistance among avian influenza A (H5N1) viruses isolated from chicken in Hebei Province of Northern China from 2001 to 2005, and investigated the amantadine use in this area. Plague reduction assay in MDCK cells showed that 83.3% isolates (5/6) were amantadine-resistant strains. The M2 sequence analysis revealed that four of five resistant isolates contained the point mutations (Ser to Asn) at position 31 that could confer resistance,to amantadine. These results indicated that the incidence of amantadine-resistant viruses isolated in Northern China was particularly high. In the investigation of amantadine use, we found that amantadine was used extensively in poultry farms in this area, which maybe was one of reasons of the high amantadine-resistance incidence. (C) 2007 Elsevier B.V. All rights reserved.