Colocalization of connexin 43 and connexin 45 but absence of connexin 40 in granulosa cell gap junctions of rat ovary.
Colocalization of connexin 43 and connexin 45 but absence of connexin 40 in granulosa cell gap junctions of rat ovary.
复制标题
大鼠卵巢颗粒细胞间隙连接中连接蛋白 43 和连接蛋白 45 共定位，但不存在连接蛋白 40。
DOI：
10.1530/jrf.0.1070255
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Y. Shibata
中科院分区：
文献类型：
--
作者：
A. Okuma;Akio Kuraoka;Hiroshi Iida;T. Inai;K. Wasano;Y. Shibata
在未刺激和促性腺激素刺激的未成熟大鼠的卵泡中检查间隙连接家族蛋白（连接蛋白）的表达和定位。免疫印迹和 RNA 印迹分析显示卵巢组织中存在连接蛋白 (Cx) 43、Cx40 和 Cx45。在这些连接蛋白中，通过免疫荧光显微镜在颗粒细胞之间以与卵泡发育阶段相关的特征性表达模式鉴定出Cx43和Cx45，而Cx40在颗粒细胞中不表达，但在卵巢基质的血管中检测到。在颗粒细胞之间间隙连接的一些斑块中，发现 Cx45 与 Cx43 共定位。在免疫荧光显微镜下，Cx43的表达随着卵泡的生长而增加，但在注射人绒毛膜促性腺激素诱导排卵后下降。相比之下，Cx45 蛋白在卵泡发育过程中持续表达；然而，排卵后，黄体中未检测到Cx45染色。讨论了 Cx43 和 Cx45 在不同发育阶段卵巢颗粒细胞细胞间通讯中的双重表达和功能作用。
The expression and localization of gap junction family proteins (connexins) were examined in nonstimulated and gonadotrophin-stimulated ovarian follicles of immature rats. Immunoblot and RNA blot analysis showed the presence of connexin (Cx) 43, Cx40 and Cx45 in ovarian tissue. Of these connexin proteins, Cx43 and Cx45 were identified by immunofluorescent microscopy between granulosa cells in characteristic expression patterns related to follicular developmental stages, while Cx40 was not expressed in granulosa cells but was detected in blood vessels in ovarian stroma. In some plaques of gap junction between granulosa cells, Cx45 was found to be colocalized with Cx43. In immunofluorescent microscopy, the expression of Cx43 was increased with follicular growth, but decreased after induction of ovulation by injection of human chorionic gonadotrophin. In contrast, the Cx45 protein was constantly expressed through follicular development; however, after ovulation, no staining of Cx45 was detected in the corpus luteum. Dual expression and the functional role of Cx43 and Cx45 in cell-to-cell communication in ovarian granulosa cells at various developmental stages were discussed.
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影响因子：
12.4
作者：
Ke, F.;Voss, A.;Kaufmann, T.
通讯作者：
Kaufmann, T.
影响因子：
20.1
作者：
KANTER, HL;LAING, JG;SAFFITZ, JE
通讯作者：
SAFFITZ, JE
表皮间隙连接中连接蛋白的发育调节和结构组织。
影响因子：
2.7
作者：
Risek,B;Klier,FG;Gilula,NB
通讯作者：
Gilula,NB
影响因子：
11.4
作者：
STEINBERG, TH;CIVITELLI, R;BEYER, EC
通讯作者：
BEYER, EC