Extremely high Langerhans cell infiltration contributes to the favourable prognosis of HPV-infected squamous cell carcinoma and adenocarcinoma of the lung
Extremely high Langerhans cell infiltration contributes to the favourable prognosis of HPV-infected squamous cell carcinoma and adenocarcinoma of the lung
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DOI：
10.1046/j.1365-2559.2001.01067.x
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发表时间：
2001-04-01
期刊：
影响因子：
6.4
通讯作者：
Hirayasu, T
中科院分区：
文献类型：
--
作者：
Miyagi, J;Kinjo, T;Hirayasu, T
目的：研究朗格汉斯细胞在肺腺癌和鳞状细胞癌中的浸润与预后和人乳头瘤病毒（HPV）感染的关系。方法与结果：选取1995 ~ 1997年腺癌62例，鳞状细胞癌59例，随访预后。使用抗S100a和CD1抗体对朗格汉斯细胞进行免疫组织化学检测。采用聚合酶链反应（PCR）和非同位素原位杂交（NISH）方法检测人乳头瘤病毒（HPV）感染。采用Kaplan-Meier法（Wilcoxon分析）和多元回归分析进行统计学分析，12例（19.4%）腺癌患者存在HPV感染。hpv感染的腺癌具有丰富的弱嗜酸性细胞质，表面活性剂载脂蛋白a免疫组化阳性。59例鳞状细胞癌中高分化19例，中分化29例，低分化11例。HPV检出29例（49.2%），其中高分化13例，中分化16例。在所有hpv感染的腺癌和鳞状细胞癌病例中，在肿瘤巢中发现了大量的朗格汉斯细胞（每个高倍视场超过100个）。相比之下，在非hpv感染的腺癌和鳞状细胞癌中，仅观察到少数朗格汉斯细胞（每高倍视场小于10个）。朗格汉斯细胞浸润高且感染HPV的鳞状细胞癌预后明显较好（P = 0.007）。高朗格汉斯细胞浸润的腺癌患者预后往往好于低朗格汉斯细胞浸润的腺癌患者，但差异无统计学意义。高度渗透案件的数量很少，不足以进行充分的统计分析。此外，在鳞状细胞癌或腺癌病例中，朗格汉斯细胞浸润与吸烟或HPV感染与吸烟之间均无显著相关性。结论：认为肿瘤中朗格汉斯细胞的高浸润是由HPV感染引起的。肿瘤中极大量的朗格汉斯细胞有助于hpv感染肺癌的良好预后。
Aims: The infiltration of Langerhans cells in adenocarcinomas and squamous cell carcinomas of the lung was examined in relation to prognostic implications and human papillomavirus (HPV) infection.Methods and results: Samples from 62 adenocarcinoma and 59 squamous cell carcinoma patients in 1995-97, the prognosis of which had been followed up, were used. The Langerhans cells were demonstrated immunohistochemically using anti S100a and CD1 antibodies. Human papillomavirus (HPV) infection was examined by polymerase chain reaction (PCR) and nonisotopic in-situ hybridization (NISH) methods. Statistical analysis was carried out using the Kaplan-Meier method (Wilcoxon analysis) and multiple regression analysis, HPV infection was demonstrated in 12 cases (19.4%) of adenocarcinoma. The HPV-infected adenocarcinomas had abundant faintly eosinophilic cytoplasm, and were immunohistochemically positive for the surfactant apoprotein A. In the 59 cases of squamous cell carcinomas 19 were of the well differentiated form, and 29 and 11 were moderately and poorly differentiated cases, respectively. HPV was detected in 29 cases (49.2%) (13 well and 16 moderately differentiated cases). In all HPV-infected adenocarcinoma and squamous cell carcinoma cases, extremely large numbers of Langerhans cells (more than 100 per high-power field) were demonstrated in the tumour nests. In contrast, in the non-HPV-infected adenocarcinomas and squamous cell carcinomas, only a few (less than about 10 per high-power field) Langerhans cells were observed. The squamous cell carcinoma cases with high Langerhans cell infiltration, which were also infected with HPV, showed a significantly good prognosis (P = 0.007). The adenocarcinoma cases with high Langerhans cell infiltration tended to have a better prognosis than the cases with low Langerhans cell infiltration, but the difference was not statistically significant. The low number of highly infiltrated cases was insufficient for an adequate statistical analysis. Furthermore, there was no significant correlation between either Langerhans cell infiltration and smoking, or HPV infection and smoking, in either squamous cell carcinoma or adenocarcinoma cases.Conclusions: It was considered that the extremely high Langerhans cell infiltration in the tumours was caused by HPV infection. The extremely large number of Langerhans cells in the tumours contributes to the favourable prognosis for HPV-infected lung cancer.