A cell-and-plasma numerical model reveals hemodynamic stress and flow adaptation in zebrafish microvessels after morphological alteration
A cell-and-plasma numerical model reveals hemodynamic stress and flow adaptation in zebrafish microvessels after morphological alteration
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DOI：
10.1101/2022.12.07.519059
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发表时间：
2022-12
影响因子：
4.3
通讯作者：
Swe Soe Maung Ye;Li-Kun Phng
中科院分区：
文献类型：
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作者：
Swe Soe Maung Ye;Li-Kun Phng
功能性心血管系统的发育确保了胚胎发育的成功，以及可持续的氧气、营养和激素输送系统，以实现成年期的体内平衡。虽然早期血管是通过生化信号和遗传编程形成的，但血流的开始提供了参与胚胎心血管系统血管重塑的机械线索。斑马鱼是一个多产的动物模型，用于研究血流和血管形态发生之间的定量关系，由于有利因素的组合，包括血流可视化在光学透明的幼虫。虽然成像技术可用于测量流速和血液灌注水平，但血液动力学力，如管腔壁剪切应力（WSS）和管腔血压不能直接测量。在这项研究中，我们已经开发了一个细胞和血浆血液传输模型，使用CFD来了解红细胞（RBC）分配不对称性如何影响网络中的WSS。此外，我们采用了CFD模型的流变学和形态学改变的野生型网络。我们讨论如何仔细考虑边界条件和采集系统参数的血流需要了解流动生理和代偿反应的血液流变学或形态学改变之间的密切关系。
The development of a functional cardiovascular system ensures successful embryonic development and a sustainable oxygen, nutrient and hormones delivery system for homeostasis in adulthood. While early vessels are formed by biochemical signaling and genetic programming, the onset of blood flow provides mechanical cues that participate in the vascular remodeling of the embryonic cardiovascular system. The zebrafish is a prolific animal model for studying the quantitative relationship between blood flow and vascular morphogenesis due to a combination of favorable factors including blood flow visualization in optically transparent larvae. While imaging techniques can be employed for measuring flow velocity and blood perfusion levels, hemodynamic forces such as the lumen wall shear stress (WSS) and lumen blood pressure cannot be measured directly. In this study, we have developed a cell and plasma blood transport model using CFD to understand how red blood cell (RBC) partitioning asymmetries affect WSS in a network. Furthermore, we employed the CFD model on rheological and morphological alterations of a wildtype network. We discuss how careful consideration of boundary conditions and acquisition of systemic parameters of blood flow are required to understand the intimate relationship between flow physiology and compensatory responses to hemorheological or morphological alterations.