Bacterial infection induces nitric oxide synthase in human neutrophils
Bacterial infection induces nitric oxide synthase in human neutrophils
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DOI：
10.1172/jci119121
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发表时间：
1997-01-01
影响因子：
15.9
通讯作者：
Sessa, WC
中科院分区：
文献类型：
--
作者：
Wheeler, MA;Smith, SD;Sessa, WC
表达诱导型一氧化氮合酶的人类炎性细胞的鉴定和诱导型一氧化氮合酶在人类感染或炎症过程中的作用的阐明一直是难以捉摸的。在来自尿液的富含亲核因子的级分中，我们证明了与来自未感染对照的富含亲核因子的级分相比，尿路感染患者中一氧化氮合酶活性增加了43倍，部分纯化的诱导型一氧化氮合酶主要是膜相关的，钙非依赖性的，并且被精氨酸类似物抑制，其等级顺序与纯化的人诱导型一氧化氮合酶的等级顺序一致。和免疫细胞化学证据明确地确定诱导型一氧化氮合酶作为主要的一氧化氮合酶亚型发现于中性粒细胞分离的尿液中，感染.在尿路感染和抗生素治疗的患者中测量的升高的诱导型一氧化氮合酶活性和升高的一氧化氮合酶蛋白直到抗生素治疗6-10 d才降低。在尿路感染期间中性粒细胞诱导型一氧化氮合酶的延长升高可能具有抗菌和促炎功能。
The identification of human inflammatory cells that express inducible nitric oxide synthase and the clarification of the role of inducible nitric oxide synthase in human infectious or inflammatory processes have been elusive, Ln neutrophil-enriched fractions from urine, we demonstrate a 43-fold increase in nitric oxide synthase activity in patients with urinary tract infections compared with that in neutrophil-enriched fractions from noninfected controls, Partially purified inducible nitric oxide synthase is primarily membrane associated, calcium independent, and inhibited by arginine analogues with a rank order consistent with that of purified human inducible nitric oxide synthase, Molecular, biochemical, and immunocytochemical evidence unequivocally identifies inducible nitric oxide synthase as the major nitric oxide synthase isoform found in neutrophils isolated from urine during urinary tract infections. Elevated inducible nitric oxide synthase activity and elevated nitric oxide synthase protein measured in patients with urinary tract infections and treated with antibiotics does not decrease until 6-10 d of antibiotic treatment, The extended elevation of neutrophil inducible nitric oxide synthase during urinary tract infections may have both antimicrobial and proinflammatory functions.