Identification and Quantification of Glucosinolates in Kimchi by Liquid Chromatography-Electrospray Tandem Mass Spectrometry
Identification and Quantification of Glucosinolates in Kimchi by Liquid Chromatography-Electrospray Tandem Mass Spectrometry
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DOI：
10.1155/2017/6753481
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发表时间：
2017-01-01
影响因子：
1.8
通讯作者：
Kim, Jang Eok
中科院分区：
文献类型：
--
作者：
Kim, Ho Jin;Lee, Mi Jin;Kim, Jang Eok
采用液相色谱-电喷雾串联质谱 (LCMS/MS) 开发了一种新颖且简单的方法来检测泡菜中的五种芥子油苷（葡糖苷素、葡糖苷、芥子油苷、芥子油素和 4-甲氧基芥子油苷）。通过比较保留时间、质谱，成功鉴定了五种芥子油苷的色谱峰。流动相由A（乙腈）和B（水）组成。至于芥子油苷，通过黑芥子苷发现了相对含量，并观察到了以前在泡菜中未发现的五种不同化合物。本研究对市场销售的20种泡菜中硫代葡萄糖苷进行了监测，考察了5种成分的不同质量和数量组成。糖胞素含量为0.00～7.07μmol/g日重(DW)，平均含量为0.86μmol/g DW；葡糖苷含量为0.00～5.85μmol/g DW，平均含量为1.17μmol/g DW。芸苔素的含量在 0.00 至 11.87 μmol/g DW 之间变化（平均值 = 3.03 μmol/g DW），而芸苔素的含量在 0.00 至 0.42 μmol/g DW 之间变化（平均值 = 0.06 μmol/g DW）。 4-甲氧基芸苔素含量范围为0.12至9.36μmol/g DW（平均值=3.52μmol/g DW）。含量比较发现，大多数情况下，4-甲氧基芸苔素的含量最高。
A novel and simple method for detecting five glucosinolates (glucoalyssin, gluconapin, glucobrassicanapin, glucobrassicin, and 4-methoxyglucobrassicin) in kimchi was developed using liquid chromatography-electrospray tandem mass spectrometry (LCMS/ MS). The chromatographic peaks of the five glucosinolateswere successfully identified by comparing their retention times, mass spectra. The mobile phase was composed of A (acetonitrile) and B (water). As for glucosinolate, the relative quantities were found through sinigrin, and five different compounds that have not been previously discovered in kimchi were observed. Monitoring was carried out on the glucosinolate in 20 kimchis distributed in markets, and this study examined the various quality and quantity compositions of the five components. The glucoalyssin content ranged from 0.00 to 7.07 mu mol/g of day weight (DW), with an average content of 0.86 mu mol/g of DW, whereas the gluconapin content ranged from 0.00 to 5.85 mu mol/g of DW, with an average of 1.17 mu mol/g of DW. The content of glucobrassicanapin varied between 0.00 and 11.87 mu mol/g of DW (average = 3.03 mu mol/g of DW), whereas that of glucobrassicin varied between 0.00 and 0.42 mu mol/g of DW (average = 0.06 mu mol/g of DW). The 4methoxyglucobrassicin content ranged from 0.12 to 9.36 mu mol/g of DW (average = 3.52 mu mol/g of DW). A comparison of the contents revealed that, in most cases, the content of 4-methoxyglucobrassicin was the highest.