SARS-CoV-2 saliva testing is a useful tool for Covid-19 diagnosis.
SARS-CoV-2 saliva testing is a useful tool for Covid-19 diagnosis.
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DOI：
10.1016/j.jviromet.2021.114241
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发表时间：
2021-10
影响因子：
3.1
通讯作者：
Levi JE
中科院分区：
文献类型：
--
作者：
de Oliveira CM;Brochi L;Scarpelli LC;Lopes ACW;Levi JE
SARS-CoV-2是2019冠状病毒病（COVID-19）的病原，根据世界卫生组织的建议，主要通过RT-PCR方法从上呼吸道标本中检测。口腔/鼻咽拭子可能使患者感到不舒服，需要训练有素的医护人员，并可能产生气溶胶，增加院内感染的风险。在这项研究中，我们描述了唾液样本中两种不提取SARS-CoV-2 RNA的单次RT-PCR方法，并将结果与来自400例患者的配对口腔/鼻咽拭子样本进行了比较。与口腔/鼻咽拭子方案相比，两种唾液方案显示出实质性的一致性。唾液协议阳性率随病程的延长而增加。其中一种唾液方案的95%检出限为9441拷贝/mL。我们的研究结果支持这样的结论，即使用自采集唾液标本进行无RNA提取的RT-PCR可以替代鼻咽拭子，特别是在症状发作的早期阶段。
SARS-CoV-2 is the etiologic agent of coronavirus disease 2019 (COVID-19) and is mainly detected by RT-PCR methods from upper respiratory specimens, as recommended by the World Health Organization. Oro/nasopharyngeal swabbing can be discomfortable to the patients, requires trained healthcare personnel and may generate aerosol, increasing the risk of nosocomial infections. In this study, we describe two SARS-CoV-2 RNA extraction-free single RT-PCR protocols on saliva samples and compared the results with the paired oro/nasopharyngeal swab specimens from 400 patients. The two saliva protocols demonstrated a substantial agreement when compared to the oro/nasopharyngeal swab protocol. Moreover, the positivity rate of saliva protocols increased according to the disease period. The 95 % limit of detection of one of the therefore implemented saliva protocol was determined as 9441 copies/mL. Our results support the conclusion that RNA extraction-free RT-PCR using self-collected saliva specimens is an alternative to nasopharyngeal swabs, especially in the early phase of symptom onset.
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影响因子：
16.6
作者：
Smyrlaki I;Ekman M;Lentini A;Rufino de Sousa N;Papanicolaou N;Vondracek M;Aarum J;Safari H;Muradrasoli S;Rothfuchs AG;Albert J;Högberg B;Reinius B
通讯作者：
Reinius B
影响因子：
14.2
作者：
Pasomsub, E.;Watcharananan, S. P.;Phuphuakrat, A.
通讯作者：
Phuphuakrat, A.
影响因子：
11.8
作者：
To, Kelvin Kai-Wang;Tsang, Owen Tak-Yin;Yuen, Kwok-Yung
通讯作者：
Yuen, Kwok-Yung
影响因子：
11.8
作者：
Yokota, Isao;Shane, Peter Y.;Teshima, Takanori
通讯作者：
Teshima, Takanori
DOI：
10.1016/j.medj.2020.12.010
发表时间：
2021-03-12
期刊：
Med (New York, N.Y.)
影响因子：
--
作者：
Vogels CBF;Watkins AE;Harden CA;Brackney DE;Shafer J;Wang J;Caraballo C;Kalinich CC;Ott IM;Fauver JR;Kudo E;Lu P;Venkataraman A;Tokuyama M;Moore AJ;Muenker MC;Casanovas-Massana A;Fournier J;Bermejo S;Campbell M;Datta R;Nelson A;Yale IMPACT Research Team;Dela Cruz CS;Ko AI;Iwasaki A;Krumholz HM;Matheus JD;Hui P;Liu C;Farhadian SF;Sikka R;Wyllie AL;Grubaugh ND
通讯作者：
Grubaugh ND