Phagocytosis of Streptococcus uberis by bovine mammary gland macrophages
Phagocytosis of Streptococcus uberis by bovine mammary gland macrophages
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DOI：
10.1016/s0034-5288(97)90184-3
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发表时间：
1997-01-01
影响因子：
2.4
通讯作者：
Finch, JM
中科院分区：
文献类型：
--
作者：
Grant, RG;Finch, JM
研究了从牛乳腺中分离的巨噬细胞吞噬和杀伤尿链球菌的能力。根据已知的对相同试验系统中牛外周血中性粒细胞吞噬作用的抗性（C197C）或敏感性（EF20）选择所用的链球菌菌株。从中期干燥期间收集的乳腺分泌物中分离的巨噬细胞能够在血清和脱脂乳存在下吞噬两种菌株。从血清中去除补体的brat灭活不影响调理素的吞噬作用的乳房巨噬细胞和IgG 1和IgG 2同种型，调理两种菌株。通过电子显微镜证实了细胞对硫代巴比妥的摄取。潜在的机制，sucrose可以抵抗中性粒细胞的吞噬作用，但不是巨噬细胞进行了讨论。
The ability of macrophages isolated from the bovine mammary gland to phagocytose and kill Streptococcus uberis was investigated. The strains of S uberis used were selected on the basis of their known resistance (C197C) or sensitivity (EF20) to phagocytosis by bovine peripheral blood neutrophils in the same assay system. Macrophages isolated from mammary secretions collected during the mid-dry period were capable of phagocytosing both strains of S uberis in the presence of serum and skimmed milk. The removal of complement from serum by brat-inactivation did not influence the opsonization of S uberis for phagocytosis by mammary macrophages and both IgG1 and IgG2 isotypes were found to opsonise both strains. The uptake of S uberis into the cells was confirmed by electron microscopy. Potential mechanisms by which S uberis could resist phagocytosis by neutrophils but not by macrophages are discussed.