Efficient and fast identification of differentially methylated regions using whole-genome bisulfite sequencing data.
Efficient and fast identification of differentially methylated regions using whole-genome bisulfite sequencing data.
复制标题
使用全基因组亚硫酸氢盐测序数据高效快速地鉴定差异甲基化区域。
DOI：
10.1016/j.jgg.2018.07.008
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Zhang,Kun
中科院分区：
文献类型：
--
作者：
Diep,Dinh;Zhang,Kun
Bisulfite padlock probes (BSPP) are a method for the targeted quantification of DNA methylation in mammalian genomes. They can simultaneously characterize the level of methylcytosine modification in a large number of targeted regions at single-base resolution. A major advantage of BSPP is that it allows the flexible capture of an arbitrary subset of genomic regions (hundreds to hundreds of thousands of genomic loci) in single-tube reactions. Large number of samples can be processed efficiently and converted into multiplexed sequencing libraries with only three enzymatic steps, without the conventional library preparation procedures. BSPP are applicable to clinical studies, screening cell lines, and for quantifying low abundance regions using deep sequencing.
