THIAMINE-DEPENDENT ACCUMULATION OF TETRAMETHYLPYRAZINE ACCOMPANYING A MUTATION IN ISOLEUCINE-VALINE PATHWAY
THIAMINE-DEPENDENT ACCUMULATION OF TETRAMETHYLPYRAZINE ACCOMPANYING A MUTATION IN ISOLEUCINE-VALINE PATHWAY
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DOI：
10.1128/jb.94.2.323-326.1967
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发表时间：
1967-01-01
影响因子：
3.2
通讯作者：
TRENNER, NR
中科院分区：
文献类型：
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作者：
DEMAIN, AL;JACKSON, M;TRENNER, NR
发现谷氨酸棒杆菌的突变体积累高浓度的物质，该物质在肉汤冷却时结晶。该化合物被鉴定为四甲基嗪。发现突变体 wss 的生长需要异亮氨酸、缬氨酸、亮氨酸和泛酸。所有四种要求可能都是由于异亮氨酸-缬氨酸途径中单一酶的缺失造成的。由于粗糙脉孢菌的类似突变体积累乙偶姻，因此本发明的突变体可能由乙偶姻形成四甲基吡嗪。四甲基吡嗪的积累取决于硫胺素的添加。这一观察结果与二磷酸硫胺作为从丙酮酸形成乙酰乳酸（乙偶姻的前体）的辅助因子的已知活性一致。
A mutant of Corynebacterium glutamicum was found to accumulate high concentrations of a material which crystallized upon cooling of the broth. The compound was identified as tetra-methylprazine. The mutant wss found to require isoleucine, valine, leucine, and pantothenate for growth. All four requirements probably result from the loss of a single enzyme of the isoleucine-valine pathway. Since similar mutants of Neurospora crassa accumulate acetoin, the present mutant probably forms tetramethylpyrazine from acetoin. Accumulation of tetramethylpyrazine was dependent upon addition of thiamine. This observation is consistent with the known activity of di-phosphothiamine as a cofactor for the formation of acetolactate (a precursor of acetoin) from pyruvate.