Identification of REO1, a gene involved in negative regulation of COX5b and ANB1 in aerobically grown Saccharomyces cerevisiae.
Identification of REO1, a gene involved in negative regulation of COX5b and ANB1 in aerobically grown Saccharomyces cerevisiae.
复制标题
REO1 的鉴定，该基因参与需氧生长的酿酒酵母中 COX5b 和 ANB1 的负调控。
DOI：
10.1093/genetics/120.3.671
复制
发表时间：
1988
期刊：
影响因子：
3.3
通讯作者：
Poyton,RO
中科院分区：
文献类型：
--
作者：
Trueblood,CE;Poyton,RO
在酿酒酵母中，COX5a和COX5b基因构成了一个小基因家族，其编码两种形式的细胞色素c氧化酶亚基V，Va和Vb，其中任一种都可以提供细胞色素c氧化酶活性和呼吸所必需的功能。在需氧生长的野生型酵母细胞中，Va是亚基V的主要形式。单独的COX5b基因不产生足够的Vb来支持足以允许在不可发酵的碳源上生长的呼吸速率。通过选择增加COX5a缺失菌株的呼吸能力的突变，我们已经鉴定了在有氧生长条件下参与COX5b表达负调控的基因。四个独立分离的reo1突变中的每一个都被证明是隐性的，与COX5b无关，但依赖于COX5b的表型表达。这些突变定义了一个单一的互补和连锁群：称为REO1，用于氧化酶表达的调节。reo1突变增加了需氧生长细胞中COX5b的表达，但在厌氧生长细胞中没有，其中表达已经升高。这些突变对COX5a没有影响，COX5a是这个小基因家族的另一个成员，它受血红素和氧的正调控。REO1基因确实在ANB 1的抑制中起作用，ANB 1是一种在有氧条件下而不是厌氧条件下通常被抑制的基因。rox1或rox3突变（先前已显示可增加ANB1表达）与reo1突变均不在同一互补组中。
In Saccharomyces cerevisiae, the COX5a and COX5b genes constitute a small gene family that encodes two forms of cytochrome c oxidase subunit V, Va and Vb, either of which can provide a function essential for cytochrome c oxidase activity and respiration. In aerobically grown wild-type yeast cells, Va is the predominant form of subunit V. The COX5b gene alone does not produce enough Vb to support a respiration rate sufficient to allow growth on nonfermentable carbon sources. By selecting for mutations that increase the respiratory capacity of a strain deleted for COX5a, we have identified a gene that is involved in negative regulation of COX5b expression under aerobic growth conditions. Each of four independently isolated reo1 mutations are shown to be recessive, unlinked to COX5b, but dependent on COX5b for phenotypic expression. The mutations define a single complementation and linkage group: designated as REO1 for regulator of expression of oxidase. reo1 mutations increase expression of COX5b in aerobically grown cells, but not in anaerobically grown cells, where expression is already elevated. These mutations have no effect on COX5a, the other member of this small gene family which is positively regulated by heme and oxygen. The REO1 gene does play a role in repression of ANB1, a gene that is normally repressed under aerobic but not anaerobic conditions. Neither rox1 or rox3 mutations, which have previously been shown to increase ANB1 expression, are in the same complementation group as reo1 mutations.