Enrichment of brain docosahexaenoic acid (DHA) is highly dependent upon the molecular carrier of dietary DHA: lysophosphatidylcholine is more efficient than either phosphatidylcholine or triacylglycerol
Enrichment of brain docosahexaenoic acid (DHA) is highly dependent upon the molecular carrier of dietary DHA: lysophosphatidylcholine is more efficient than either phosphatidylcholine or triacylglycerol
复制标题
DOI：
10.1016/j.jnutbio.2019.108231
复制
发表时间：
2019-12-01
影响因子：
5.6
通讯作者：
Subbaiah, Papasani V.
中科院分区：
文献类型：
--
作者：
Sugasini, Dhavamani;Yalagala, Poorna C. R.;Subbaiah, Papasani V.
二十二碳六烯酸（DHA）高度集中在大脑中，它的缺乏与包括阿尔茨海默病在内的几种神经系统疾病有关。然而，目前使用的补充剂并没有明显地丰富大脑DHA，尽管它们丰富了大多数其他组织。我们测试了这一假设，即饮食载体增加脑DHA的能力取决于DHA-溶血磷脂酰胆碱（LPC）的产生，这是DHA穿过血脑屏障的首选载体。我们比较了DHA-三酰甘油（TAG）、二DHA磷脂酰胆碱（PC）和DHA-LPC在正常大鼠灌胃30天后富集脑DHA的功效，所有剂量均为10 mg DHA/天。结果表明，来自TAG的DHA在消化过程中以游离DHA或单酰基甘油的形式释放，并在乳糜微粒中以TAG的形式吸收，优先掺入脂肪组织和心脏，但不掺入大脑。相比之下，LPC-DHA使大脑DHA增加了100%，但对脂肪组织没有影响。Di-DHA PC在消化过程中产生游离DHA和LPC-DHA，在大脑、心脏和肝脏中富集DHA。脑源性神经营养因子的含量在双DHA PC和DHA-LPC中有所增加，而在TAG-DHA中则没有，表明脑中DHA的富集与其功能效应有关。我们得出的结论是，来自TAG或天然PC（sn-2位）的膳食DHA不适合大脑富集，而来自LPC（在sn-1或sn-2位）或来自PC的sn-1位的DHA有效地富集了大脑并且在功能上有效。爱思唯尔公司出版
Docosahexaenoic acid (DHA) is highly concentrated in the brain, and its deficiency is associated with several neurological disorders including Alzheimer's disease. However, the currently used supplements do not appreciably enrich brain DHA, although they enrich most other tissues. We tested the hypothesis that the ability of the dietary carrier to augment brain DHA depends upon the generation of DHA-lysophosphatidylcholine (LPC), the preferred carrier of DHA across the blood brain barrier. We compared the efficacy of DHA-triacylglycerol (TAG), di-DHA phosphatidylcholine (PC) and DHA-LPC to enrich brain DHA following their gavage to normal rats for 30 days, all at a dose of 10 mg DHA/day. The results show that DHA from TAG, which is released as free DHA or monoacylglycerol during digestion and is absorbed as TAG in chylomicrons, was incorporated preferentially into adipose tissue and heart but not into brain. In contrast, LPC-DHA increased brain DHA by up to 100% but had no effect on adipose tissue. Di-DHA PC, which generates both free DHA and LPC-DHA during the digestion, enriched DHA in brain, as well as in heart and liver. Brain-derived neurotrophic factor was increased by di-DHA PC and DHA-LPC, but not by TAG-DHA, showing that enrichment of brain DHA correlated with its functional effect. We conclude that dietary DHA from TAG or from natural PC (sn-2 position) is not suitable for brain enrichment, whereas DHA from LPC (at either sn-1 or sn-2 position) or from sn-1 position of PC efficiently enriches the brain and is functionally effective. Published by Elsevier Inc.