Slow and steady wins the race: physical limits on the rate of viral DNA packaging
Slow and steady wins the race: physical limits on the rate of viral DNA packaging
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DOI：
10.1016/j.coviro.2019.03.002
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发表时间：
2019-06-01
影响因子：
5.9
通讯作者：
Jardine, Paul J.
中科院分区：
文献类型：
--
作者：
Jardine, Paul J.
在双链DNA病毒（如尾噬菌体和疱疹病毒）的组装过程中，病毒染色体在预先形成的头壳内被压缩至接近晶体密度。DNA易位由强大的环状ATP酶马达驱动，该马达将ATP结合、水解和释放耦合到力的产生和运动。对噬菌体phi29马达的研究揭示了这一过程背后的复杂机械化学，随着头部填充而减慢。最近对包装DNA的物理行为的研究表明，头部内DNA的长时间松弛和包装过程中的干扰现象产生了调节包装速率的物理需要。因此，对病毒系统中DNA包装的研究揭示了对DNA复杂行为的基本认识，以及生物系统适应这些物理约束的必要性。
During the assembly of dsDNA viruses such as the tailed bacteriophages and herpesviruses, the viral chromosome is compacted to near crystalline density inside a preformed head shell. DNA translocation is driven by powerful ring ATPase motors that couple ATP binding, hydrolysis, and release to force generation and movement. Studies of the motor of the bacteriophage phi29 have revealed a complex mechanochemistry behind this process that slows as the head fills. Recent studies of the physical behavior of packaging DNA suggest that surprisingly long-time scales of relaxation of DNA inside the head and jamming phenomena during packaging create the physical need for regulation of the rate of packaging. Studies of DNA packaging in viral systems have, therefore, revealed fundamental insight into the complex behavior of DNA and the need for biological systems to accommodate these physical constraints.