Kinetic analysis of translocation through nuclear pore complexes
Kinetic analysis of translocation through nuclear pore complexes
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DOI：
10.1093/emboj/20.6.1320
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发表时间：
2001-03-15
期刊：
影响因子：
11.4
通讯作者：
Görlich, D
中科院分区：
文献类型：
--
作者：
Ribbeck, K;Görlich, D
通过核孔复合体促进易位的机制还知之甚少。在这里，我们提出了一个使用各种模型底物的过程的动力学分析。我们发现，鼻咽癌的易位能力出人意料地高，单个鼻咽癌允许近100mda/S的质量流量，速率约为每秒10(3)个易位事件。我们的数据进一步表明，易位底物和NPC组分之间的高亲和力相互作用对于易位是必不可少的。我们提出了一个“选择性阶段模型”，该模型可以解释NPC如何作为惰性分子的通透屏障，但又对核运输受体和受体-货物复合体具有选择性的通透性。
The mechanism of facilitated translocation through nuclear pore complexes (NPCs) is only poorly understood. Here, we present a kinetic analysis of the process using various model substrates. We find that the translocation capacity of NPCs is unexpectedly high, with a single NPC allowing a mass flow of nearly 100 MDa/s and rates in the order of 10(3) translocation events per second. Our data further indicate that high affinity interactions between the translocation substrate and NPC components are dispensable for translocation, We propose a 'selective phase model' that could explain how NPCs function as a permeability barrier for inert molecules and yet become selectively permeable for nuclear transport receptors and receptor-cargo complexes.