Structured illumination for wide-field Raman imaging of cell membranes
Structured illumination for wide-field Raman imaging of cell membranes
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用于细胞膜宽视场拉曼成像的结构照明
DOI：
10.1016/j.optcom.2017.06.021
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发表时间：
2017-11-01
影响因子：
2.4
通讯作者：
Yuan, Xiaocong
中科院分区：
文献类型：
--
作者：
Chen, Houkai;Wang, Sigi;Yuan, Xiaocong
尽管衍射极限仍然限制了其横向分辨率，但与扫描方案相比，传统的宽场拉曼成像技术提供了较快的成像速度。为了利用滤光片提高宽场拉曼显微镜的横向分辨率，采用了驻波照明技术，实现了横向分辨率提高两倍以上。具体而言，使用功能化的表面增强拉曼散射纳米粒子来增强所需的散射信号以标记细胞膜。这种宽场拉曼成像技术在生物应用中提供了各种重要的机会。（C）2017爱思唯尔有限公司。保留所有权利。
Although the diffraction limit still restricts their lateral resolution, conventional wide-field Raman imaging techniques offer fast imaging speeds compared with scanning schemes. To extend the lateral resolution of wide field Raman microscopy using filters, standing-wave illumination technique is used, and an improvement of lateral resolution by a factor of more than two is achieved. Specifically, functionalized surface enhanced Raman scattering nanoparticles are employed to strengthen the desired scattering signals to label cell membranes. This wide-field Raman imaging technique affords various significant opportunities in the biological applications. (C) 2017 Elsevier B.V. All rights reserved.