Regulated upon activation normal T-cell expressed and secreted originating from the epididymis differentially associates with viable and defective spermatozoa
Regulated upon activation normal T-cell expressed and secreted originating from the epididymis differentially associates with viable and defective spermatozoa
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源自附睾的正常 T 细胞表达和分泌受激活调节，与活精子和有缺陷精子有不同关联
DOI：
10.1016/j.fertnstert.2010.01.053
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发表时间：
2010-05-15
影响因子：
6.7
通讯作者：
Zhang, Yuan-qiang
中科院分区：
文献类型：
--
作者：
Li, Zhen;Sun, Zhi-jian;Zhang, Yuan-qiang
目的：探讨激活后表达和分泌的调节性T细胞（RANTES）在男性生殖系统中的表达和细胞分布。设计：基础研究。地点：大学学术医学中心。患者：3名成年男性器官捐献者。干预措施：无。主要指标：采用逆转录聚合酶链式反应（RT-PCR）、原位杂交、免疫组化染色和免疫荧光染色检查RANTES 在人和小鼠附睾中的分布。使用蛋白质印迹法定量出生后几天小鼠附睾中 RANTES 表达水平。应用免疫荧光染色检测RANTES与小鼠附睾精子的关联。结果：在人和小鼠中，RANTES的位置仅限于传出管的纤毛细胞以及附睾管的顶端、狭窄和基底细胞。 RANTES 阳性基底细胞仅在人类附睾管中发现。 RANTES 信号在第 28 天首次检测到，并在小鼠性成熟过程中增强。我们还观察到 RANTES 与正常和有缺陷的附睾精子结合，但以不同的模式。结论：RANTES 在附睾中组成型表达，并在整个性成熟过程中分泌到附睾管腔中，并与有活力和有缺陷的精子有不同的关联。 （Fertil Steril (R) 2010；93: 2661-7。(C) 2010，美国生殖医学会。）
Objective: To investigate the expression and cellular distribution of regulated upon activation normal T-cell expressed and secreted (RANTES) in the male reproductive system.Design: Basic research.Setting: University academic medical center.Patient(s): Three adult male organ donors.Intervention(s): None.Main Outcome Measure(s): Reverse transcriptase-polymerase chain reaction (RT-PCR), in situ hybridization, immunohistochemical staining, and immunofluorescence staining were used to examine the distribution of RANTES in human and mouse epididymis. Western blot was used to quantitate the levels of RANTES expression in mouse epididymis on postnatal days. Immunofluorescence staining was applied to detect RANTES association with spermatozoa from mouse epididymis.Result(s): The location of RANTES was restricted to ciliated cells of the efferent duct and apical, narrow, and basal cells of the epididymal ducts, in both humans and mouse. RANTES-positive basal cells were only identified in the epididymal ducts in humans. The signals of RANTES were first detected on day 28 and increased during mouse sexual maturation. We also observed that RANTES was bound on both normal and defective epididymal sperm, but in different patterns.Conclusion(s): RANTES is constitutively expressed in the epididymis and secreted into the lumen of epididymis throughout sexual maturity, and differentially associates with viable and defective spermatozoa. (Fertil Steril (R) 2010; 93: 2661-7. (C) 2010 by American Society for Reproductive Medicine.)