A CD52 antibody impairs mouse-transplanted intestinal tight junctions.
A CD52 antibody impairs mouse-transplanted intestinal tight junctions.
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DOI：
10.1016/j.jss.2015.02.051
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发表时间：
2015-06
期刊：
影响因子：
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通讯作者：
B. Shen;Hong-Mei Yu;Tunan Yu;Jiliang Shen;N. Meng;Xiujun Cai
中科院分区：
文献类型：
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作者：
B. Shen;Hong-Mei Yu;Tunan Yu;Jiliang Shen;N. Meng;Xiujun Cai
背景使用CD 52单克隆抗体（mAb）的诱导免疫抑制策略已被引入小肠移植，导致改善的结果。然而，关于抗体对肠道屏障微观结构的影响的信息很少，肠道屏障的功能是防止细菌移位。在这项研究中，我们使用了小鼠原位小肠移植模型，以调查的紧密连接（TJs），这被认为是肠屏障的中心部分，transplanted intestinal.Materials和methodsC 57 BL/6小鼠作为受体的CD 52单克隆抗体的影响。同种异体移植物取自BALB/c小鼠，同基因组取自C57 BL/6小鼠。应用抗小鼠CD 52 mAb作为替代抗体。透射电镜观察TJ超微结构。Western blot分析TJ蛋白occludin和ZO-1的表达。免疫荧光法观察TJ蛋白的分布，用FITC-dextran法检测移植肠的通透性。此外，TJ蛋白（occludin和ZO-1）表达减少，肠通透性增加。
BackgroundInduction immunosuppression strategies using a CD52 monoclonal antibody (mAb) have been introduced for small bowel transplantation, resulting in improved outcomes. However, little information is known about the effects of the antibody on the microstructure of the intestinal barrier, which functions to prevent bacterial translocation. In this study, we used a murine orthotopic small bowel transplantation model to investigate the impact of a CD52 mAb on tight junctions (TJs), which are considered a central part of the intestinal barrier, of the transplanted intestine.Materials and methodsC57BL/6 mice were used as recipients. The grafts were harvested from BALB/c mice in allogeneic groups and C57BL/6 mice in syngeneic groups. The anti-mouse CD52 mAb was applied as a surrogate antibody. Transmission electron microscopy was used to evaluate the TJ ultrastructure. The expression of the TJ proteins occludin and ZO-1 was analyzed by Western blot. The distribution of TJ proteins was observed by immunofluorescence, and the permeability of the transplanted intestine was assessedin vivousing FITC-dextran.ResultsAfter CD52 mAb application, a compromised TJ ultrastructure was observed. In addition, TJ protein expression (occludin and ZO-1) decreased and the intestinal permeability increased.ConclusionsThe anti-mouse CD52 mAb impaired the transplanted intestinal TJ and barrier.