In vivo interaction between Tobacco mosaic virus RNA-dependent RNA polymerase and host translation elongation factor 1A.
In vivo interaction between Tobacco mosaic virus RNA-dependent RNA polymerase and host translation elongation factor 1A.
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DOI：
10.1016/j.virol.2005.11.031
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发表时间：
2006-03
期刊：
影响因子：
3.7
通讯作者：
Y. Yamaji;Toshihiko Kobayashi;K. Hamada;K. Sakurai;A. Yoshii;Masashi Suzuki;S. Namba;T. Hibi
中科院分区：
文献类型：
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作者：
Y. Yamaji;Toshihiko Kobayashi;K. Hamada;K. Sakurai;A. Yoshii;Masashi Suzuki;S. Namba;T. Hibi
一些宿主翻译延伸因子已经被认为在植物、动物和细菌中病毒RNA的复制和翻译中起重要作用。在这里，我们显示了真核翻译延伸因子1A（eEF 1A）和烟草花叶病毒（TMV）的RNA依赖的RNA聚合酶（RdRp）在体内的相互作用，通过免疫沉淀。烟草eEF 1A不仅与TMV RNA的3′-非翻译区（3′-UTR）相互作用，而且不经3′-UTR介导，直接与RdRp相互作用。TMV RdRp的甲基转移酶结构域在体外和酵母中与eEF 1A相互作用。这些结果表明eEF 1A是TMV病毒复制复合物的一个组分。
Several host translation elongation factors have been suggested to play essential roles in the replication and translation of viral RNAs in plants, animals and bacteria. Here, we show the interaction between eukaryotic translation elongation factor 1A (eEF1A) and Tobacco mosaic virus (TMV) RNA-dependent RNA polymerase (RdRp) in vivo by immunoprecipitation. The tobacco eEF1A interacted not only with 3′-untranslated region (3′-UTR) of TMV RNA but also directly with RdRp without mediation by the 3′-UTR. The methyltransferase domain of TMV RdRp was indicated to be responsible for the interaction with eEF1A in vitro and in yeast. These results suggest that eEF1A is a component of the virus replication complex of TMV.