Peptidoglycan recognition protein (PGRP) from eri-silkworm, Samia cynthia ricini;: protein purification and induction of the gene expression
Peptidoglycan recognition protein (PGRP) from eri-silkworm, Samia cynthia ricini;: protein purification and induction of the gene expression
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DOI：
10.1016/j.cbpb.2007.03.005
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发表时间：
2007-07-01
影响因子：
2.2
通讯作者：
Morishima, Isao
中科院分区：
文献类型：
--
作者：
Onoe, Hiroko;Matsumoto, Akiyoshi;Morishima, Isao
从免疫的野桑蚕血淋巴中分离出肽聚糖识别蛋白（PGRP），用I-125标记的肽聚糖（PGN）检测其结合活性。通过大量过量的未标记的PGN或其他葡聚糖竞争性抑制与I-125标记的PGN的结合来测试PGRP的结合特异性。同样结构的未标记PGN和芽孢杆菌细胞壁的meso-二氨基庚二酸（DAP）型交联PGN强烈抑制标记的未交联的藤黄微球菌Lys型PGN与其结合，而M.黄体细胞壁PGRP cDNA编码193个氨基酸的开放阅读框。推导的氨基酸序列与已知的鳞翅目昆虫PGRPs的同源性为62%~91%，而与果蝇PGRPs的同源性低于40%。PGRP基因在天然脂肪体中以低水平组成性表达，并受到注射DAP型交联和LyS型未交联PGN的强烈诱导，但仅受到来自M的LyS型交联PGN的弱诱导。黄体。家蚕可能基于交联肽的结构来区分PGN，但对茎肽的二氨基酸残基具有较少的偏好（如果有的话）。(c)2007年爱思唯尔公司All rights reserved.
Peptidoglycan recognition protein (PGRP) was isolated from immunized hemolymph of the wild silkworm, Samia cynthia ricini, detecting the biding activity with I-125-labeled peptidoglycan (PGN). The binding specificity of PGRP was tested by competitive inhibition of the binding to I-125-labeled-PGN by a large excess amount of non-labeled-PGN or other glucans. The binding to labeled uncross-linked Lys-type PGN from Micrococcus luteus was strongly inhibited by non-labeled-PGN of the same structure and meso-diaminopimelic acid (DAP)-type cross-linked PGN from Bacillus cell wall, but only a little by cross-linked PGN from M. luteus cell wall. The PGRP cDNA encodes a 193 amino acid open reading frame. The deduced amino acid sequence had 62 to 91% identities to known lepidopteran PGRPs, but less than 40% to Drosophila PGRPs. The PGRP gene constitutively expressed at a low level in naive fat body, and strongly induced by an injection of DAP-type cross-linked and Lys-type uncross-linked PGNs, but only weakly by Lys-type cross-linked PGN from M. luteus. The silkworm possibly distinguish between PGNs based on the structure of cross-linking peptide, but has less if any preference for the diamino acid residue of the stem peptide. (c) 2007 Elsevier Inc. All rights reserved.