MITOGENICITY OF HUMAN-FIBROBLASTS INVIVO FOR HUMAN-BREAST CANCER-CELLS
MITOGENICITY OF HUMAN-FIBROBLASTS INVIVO FOR HUMAN-BREAST CANCER-CELLS
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DOI：
10.1002/bjs.1800740326
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发表时间：
1987-03-01
影响因子：
9.6
通讯作者：
MANSEL, RE
中科院分区：
文献类型：
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作者：
HORGAN, K;JONES, DL;MANSEL, RE
基质因子与乳腺上皮细胞行为的调控有关，但其对人类乳腺癌的影响尚不清楚。以良性、恶性乳腺组织和正常皮肤为外植体，在单层细胞培养中培养成纤维细胞。将MCF7人乳腺癌细胞单独（2 × 106个细胞）或与活成纤维细胞（MCF7 + F）联合接种于6 - 8周龄雌性裸鼠乳腺脂肪垫。5周后切除肿瘤，称重并固定作组织学检查。活乳腺成纤维细胞的添加显著增加了后续肿瘤的复归率（127/135比52/106，P< 0.01， χ2检验）和生长（肿瘤重174·11比41.4 mg，平均值±s.e.m，P< 0.01， Mann-Whitney U检验）。组织学检查未发现两组肿瘤的形态学差异。单独注射成纤维细胞不产生肿瘤。皮肤成纤维细胞的添加也具有刺激作用。戊二醛杀死的成纤维细胞增加了肿瘤的摄取，但没有刺激肿瘤的生长。单核血白细胞无效。结果表明，在该模型中，成纤维细胞对人乳腺癌异种移植物的生长发育具有刺激作用。
Stromal factors have been implicated in the regulation of mammary epithelial cell behaviour, but their influence on human breast cancer is unknown. Fibroblasts were grown in monolayer cell culture from explants of benign and malignant breast tissue and normal skin. MCF7 human breast cancer cells alone (2 × 106cells) or in combination with live fibroblasts (MCF7 + F) were inoculated into the mammary fat pads of 6–8-week-old female nude mice. After 5 weeks tumours were excised, weighed and fixed for histological examination. Addition of live breast fibroblasts significantly increased the retrieval (127/135 versus 52/106, P <0·01, χ2test) and growth (tumour weight 174·11 versus 41·4 mg, mean ± s.e.m.,P<0·01, Mann—Whitney U test) of subsequent tumours. Histological examination did not reveal any morphological difference between the two tumour groups. Injection of fibroblasts alone did not produce tumours. Addition of skin fibroblasts was also stimulatory. Glutaraldehyde-killed fibroblasts increased tumour take but did not stimulate tumour growth. Mononuclear blood leucocytes were ineffective. The results demonstrate that, in this model, fibroblasts exert stimulatory influences on human breast cancer xenograft growth and development.