Calcium modulates cornified envelope formation, involucrin content, and transglutaminase activity in cultured human ectocervical epithelial cells.
Calcium modulates cornified envelope formation, involucrin content, and transglutaminase activity in cultured human ectocervical epithelial cells.
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钙调节培养的人宫颈外上皮细胞中的角质化包膜形成、外皮蛋白含量和转谷氨酰胺酶活性。
DOI：
10.1006/excr.1993.1061
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发表时间：
1993
影响因子：
3.7
通讯作者：
Rorke,EA
中科院分区：
文献类型：
--
作者：
Kasturi,L;Sizemore,N;Eckert,RL;Martin,K;Rorke,EA
宫颈管的入口，即外宫颈，被复层鳞状上皮覆盖。在本研究中，我们证明了细胞外钙水平调节培养的人宫颈上皮细胞（ECE细胞）的分化。在含有0.09 mM钙的无血清培养基中生长的ECE细胞保持为单个细胞，其不在培养皿上扩散或形成皮质包膜。增加细胞外钙浓度导致ECE细胞分化的剂量依赖性增加，这表现为皮层包膜（表面细胞）形成的增加。这些形态学变化伴随着外皮蛋白水平的两倍增加，外皮蛋白是皮层包膜的前体。外皮蛋白mRNA的增加程度与外皮蛋白蛋白的增加程度相似，表明调控是在外皮蛋白mRNA的合成和/或稳定性水平上。转氨酶是一种钙激活的酶，负责组装形成皮质包膜的共价交联，其增加程度与皮质包膜形成的增加程度相似，这表明在ECE细胞中，转氨酶的激活可能是包膜组装的限速步骤。这些结果以及在体内其他分层上皮中发现的钙梯度，表明钙可能是体外宫颈上皮细胞分化的重要调节因子。
The entrance to the cervical canal, the ectocervix, is covered by a stratified squamous epithelium. In the present study we demonstrate that the extracellular calcium levels regulate the differentiation of cultured human ectocervical epithelial cells (ECE cells). ECE cells growing in serum-free medium containing 0.09 mMcalcium remain as individual cells that do not spread on the culture dish or form cornified envelopes. Increasing the extracellular calcium concentration results in a dose-dependent increase in ECE cell differentiation that is manifest by an increase in cornified envelope (superficial cell) formation. These morphological changes are accompanied by a twofold increase in the levels of involucrin, a precursor of the cornified envelope. The extent of increase in involucrin mRNA is similar to that of involucrin protein, suggesting that the regulation is at the level of involucrin mRNA synthesis and/or stability. Transglutaminase, a calcium-activated enzyme responsible for assembly of the covalent cross-links that form the cornified envelope, increases to a similar extent to the increase in cornified envelope formation, suggesting that in ECE cells, activation of transglutaminase may be the rate-limiting step in envelope assembly. These results and the finding of a calcium gradient in other stratifying epitheliain vivo, indicate that the calcium is likely to be an important regulator of ectocervical epithelial cell differentiationin vivo.