Diverse strategies conferring extreme cadmium (Cd) tolerance in the dark septate endophyte (DSE), Exophiala pisciphila: Evidence from RNA-seq data
Diverse strategies conferring extreme cadmium (Cd) tolerance in the dark septate endophyte (DSE), Exophiala pisciphila: Evidence from RNA-seq data
复制标题
多种策略赋予深色有隔内生菌 (DSE) Exophiala pisciphila 极高的镉 (Cd) 耐受性：来自 RNA-seq 数据的证据。
DOI：
10.1016/j.micres.2014.09.005
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发表时间：
2015-01-01
影响因子：
6.7
通讯作者：
Zhao, Zhiwei
中科院分区：
文献类型：
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作者：
Zhao, Dake;Li, Tao;Zhao, Zhiwei
暗隔膜内生菌(DSE)广泛存在于极端重金属(HMS)污染土壤中的植物根部。关于DSE对过量HMS的整体分子反应知之甚少。因此，基于DSE菌株Exophiala pisciPhila的两个cDNA文库，通过Illumina HiSeq 2000测序，对无镉和Cd胁迫条件下培养的DSE菌株进行了RNA-seq序列分析，共产生21,376个非基因菌株。共获得575个差异表达基因(Deg)。大约40%的DEGS(228个非遗传基因)参与了10条已知的耐Cd(Cd)途径，包括金属离子结合和转运、有机酸代谢和运输、清除ROS、氧化还原动态平衡、转录因子产生、硫酸盐同化、DNA修复和细胞壁完整性维持等。这项研究是对Cd胁迫下DSE转录组的首次研究，我们的结果为未来真菌对HMS耐性的分子研究提供了有价值的信息。(C)2014年爱思唯尔股份有限公司。版权所有。
Dark septate endophytes (DSE) ubiquitously colonize the roots of plants growing in extreme heavy metals (HMs)-contaminated soils. Little is known about the overall molecular response of DSE to excessive HMs. Therefore, RNA-seq was performed through Illumina Hiseq 2000 sequencing based on two cDNA libraries of the DSE strain Exophiala pisciphila, cultured under cadmium (Cd)-free and Cd-stressed conditions, and 21,376 unigenes were generated. In total, 575 differentially expressed genes (DEGs) were obtained. Approximately 40% of the DEGs (228 unigenes) were involved in 10 well-known HMs-tolerant pathways, conferring the extreme cadmium (Cd) tolerance of E. pisciphila, including metal ion binding and transportation, organic acid metabolism and transportation, reactive oxygen species (ROS) scavenging, redox homeostasis, transcription factors production, sulfate assimilation, DNA repair and cell wall integrity maintenance, etc. Our results indicate that integral tactics associated with the collaboration of extracellular and intracellular mechanisms contribute to the enhanced HMs tolerance of this fungus. This study represents the first investigation of the transcriptome of DSE under Cd stress, and our results provide valuable information for future molecular studies of HMs tolerance in fungi. (C) 2014 Elsevier GmbH. All rights reserved.