Evidence for the continuation of meiosis and spermiogenesis in in vitro cultures of spermatogenic cells from Xenopus laevis
Evidence for the continuation of meiosis and spermiogenesis in in vitro cultures of spermatogenic cells from Xenopus laevis
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非洲爪蟾生精细胞体外培养物继续减数分裂和精子发生的证据
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
R. Eckhardt
中科院分区：
文献类型：
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作者：
M. S. Risley;R. Eckhardt
从非洲爪蟾睾丸中获得的生精细胞的胶原酶解离，随后通过密度梯度离心Metrizamide和单位重力沉降在牛血清白蛋白梯度分离成两个部分。一个馏分是高度富集初级精母细胞，而其他馏分是高度富集早中期精子细胞。将来自每个级分的细胞分别在改良的DNOM培养基中培养23天，以确定在体外可以维持分化的程度。差异细胞计数显示，早期中期精子细胞继续通过细胞质脱落阶段在体外分化。精母细胞在整个23天期间通过两次减数分裂，产生精子细胞，精子细胞也通过细胞质脱落阶段分化。结果表明，非洲爪蟾生精细胞在体外培养过程中，即使经过分离和细胞分离程序，仍具有明显的分化潜能。
Spermatogenic cells were obtained from Xenopus laevis testes by collagenase dissociation and subsequently separated into two fractions by density gradient centrifugation in Metrizamide and by unit gravity sedimentation in bovine serum albumin gradients. One fraction was highly enriched for primary spermatocytes while the other fraction was highly enriched for early-mid stage spermatids. The cells from each fraction were cultured separately in modified DNOM medium for 23 days to determine the extent to which differentiation could be maintained in vitro. Differential cell counts revealed that early-mid stage spermatids continues to differentiate in vitro through the stages of cytoplasmic shedding. Spermatocytes passed through both meiotic divisions throughout the 23 day period and produced spermatids which also differentiated through the cytoplasmic shedding stages. The results demonstrated that significant differentiative potential is retained by Xenopus spermatogenic cells cultured in vitro even after dissociation and cell separation procedurs.